Table 2 Genetic markers, primer sequences, and primer-specific annealing temperatures (Ta) and MgCl2 concentrations
Marker | Primer names | Primer sequences (5'-3') | Ta (°C) | MgCl2 (mM) | References |
|---|---|---|---|---|---|
COI | LCO1490 HCO2198 | TAAACTTCAGGGTGACCAAAAAATCA GGTCAACAAATCATAAAGATATTGG | 50 | 6 | [91] [91] |
ANT | StolidoANT-F ANTr1 | CAGGGTATCATTGTRTACMGAG CCAGACTGCATCATCATKCGRCGDC | 60 | 3 | This study [44] |
ATPSα | ATPSαf1 ATPSαr1 | GAGCCMATGCAGACTGGTATTAAGGCYGT CTGTGGTAGTAGTTGGTCTTCKCNAAGTT | 55 | 3 | [44] [44] |
18S | 5'F 557F 1262R 3'R | TYCCTGGTTGATYYTGCCAG GCCAGCAGCCGCGGT GGTGGTGCATGGCCGTY TGATCCATCTGCAGGTYCACCT | 54 | 3 | [92] [93] [93] [92] |