Figure 4

Histone deployment throughout O. dioica development. Developmental times are indicated with reference to predominance of different cell cycle types (bottom). Cartoons (top) illustrate potential combinatorial possibilities of histone core (wedges: H4s grey, H3s yellow, H2As blue, H2Bs green), and linker H1 (rectangles: orange to red) variants through development. Histone transcripts are represented as lines with thickened portions indicating significant increases either through greater transcriptional activity of a given gene or contributions from additional genes encoding the same variant. The latter was observed for canonical histones of all types during organogenesis with activation of additional genes. All core and linker histones are represented by at least one male-specific isoform (at right).