Evolutionary factors affecting Lactate dehydrogenase A and B variation in the Daphnia pulexspecies complex

Table 2 Nucleotide diversity statistics for LdhA and LdhB sequences from the Daphnia pulicaria (C) and Daphnia pulex (X) allele groups.

Allele Group ¹	Total length ²	Exon length ²	Intron length ²	N ³	K ⁴	K_ex⁴	K_aa⁴	H ⁵	S ⁶	π_T⁷	π_n⁷	π_s⁷	π_i⁷	θ_T⁸	θ_n⁸	θ_s⁸	θ_i⁸
LdhA-X^S	1394	981	413	75	44	32	5	0.961	147	0.0103	0.0002	0.0267	0.0217	0.0126	na⁹	na⁹	0.0269
LdhA-C^F	1394	981	413	42	15	8	4	0.833	29	0.002	0.0003	0.0061	0.0031	0.0045	0.0013	0.011	0.0072
LdhB-X	1545	972	573	71	53	49	16	0.99	199	0.0139	0.0021	0.0312	0.0245	0.0161	0.0039	0.029	0.0294
LdhB-C	1545	972	573	59	39	23	13	0.968	105	0.0071	0.0024	0.007	0.0142	0.0106	0.0044	0.0166	0.0173

1. S and F refer to slow and fast electrophoretic mobility, respectively
2. length in nucleotides based on the total alignment for each gene
3. N = the number of sequences in the allele group, including duplicates. This group of sequences was used to estimate all diversity parameters.
4. K = the number of unique sequences with indels excluded. ex = unique exon sequences, aa = unique amino acid sequences
5. H = haplotype diversity calculated from N sequences with indels excluded
6. S = the number of segregating sites calculated from N sequences with indels excluded
7. π = nucleotide diversity calculated from N sequences with pairwise deletion of indels and Jukes-Cantor correction. T = total sites, n = non-synonymous sites, s = synonymous sites, i = intron sites
8. θ = number of segregating sites calculated from N sequences with pairwise deletion of indels and Jukes-Cantor correction. T = total sites, n = non-synonymous sites, s = synonymous sites, i = intron sites
9. na = not applicable. θ is not estimated when codons differ by multiple changes

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