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Figure 3 | BMC Evolutionary Biology

Figure 3

From: Why small males have big sperm: dimorphic squid sperm linked to alternative mating behaviours

Figure 3

Fertilization success by consort and sneaker sperm. (a-c) Mature oocytes were inseminated without sperm as a negative control (a), with sperm from consort spermatophores (b) and from sneaker spermatophores (c), and then cultured 48 hours and stained with DAPI. (d) A DIC image of developing embryos after 24 hours of insemination: arrowheads point to the animal pole where cleavage planes appear along with the animal-vegetal axis. (e, f) Fertilization rates in oocytes from eight females (1-8) inseminated with sperm from consort (e) or sneaker (f) spermatophores. Each colour-coded line represents one male.

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