- Research article
- Open Access
Genomic, RNA, and ecological divergences of the Revolvertransposon-like multi-gene family in Triticeae
© Tomita et al; licensee BioMed Central Ltd. 2011
- Received: 23 March 2011
- Accepted: 25 September 2011
- Published: 25 September 2011
Revolver is a newly discovered multi-gene family of transposable elements in the Triticeae genome. Revolver encompasses 2929 to 3041 bp, has 20 bp of terminal inverted repeated sequences at both ends, and contains a transcriptionally active gene encoding a DNA-binding-like protein. A putative TATA box is located at base 221, with a cap site at base 261 and a possible polyadenylation signal AATAAA at base 2918. Revolver shows considerable quantitative variation in wheat and its relatives.
Revolver cDNAs varied between 395 and 2,182 bp in length. The first exon exhibited length variation, but the second and third exons were almost identical. These variants in the Revolver family shared the downstream region of the second intron, but varied structurally at the 5' first exon. There were 58 clones, which showed partial homology to Revolver, among 440,000 expressed sequence tagged (EST) clones sourced from Triticeae. In these Revolver homologues with lengths of 360-744 bp, the portion after the 2nd exon was conserved (65-79% homology), but the 1st exon sequences had mutually low homology, with mutations classified into 12 types, and did not have EST sequences with open reading frames (ORFs). By PCR with the 3'-flanking region of a typical genomic clone of Revolver-2 used as a single primer, rye chromosomes 1R and 5R could be simultaneously identified. Extensive eco-geographic diversity and divergence was observed among 161 genotypes of the single species Triticum dicoccoides collected from 18 populations in Israel with varying exposures to abiotic and biotic stresses (soil, temperature, altitude, water availability, and pathogens).
On the base of existing differences between Revolver variants, the molecular markers that can distinguish different rye chromosomes were developed. Eco-geographic diversification of wild emmer T. dicoccoides in Israel and high Revolver copy numbers are associated with higher rainfall and biotic stresses. The remarkable quantitative differences among copy numbers of Revolver in the same species from different ecosystems suggest strong amplification activity within the last 10,000 years. It is the interesting finding because the majority of Triticeae high-copy transposable elements seem to be inactive at the recent time except for BARE-1 element in Hordeum and the fact might be interesting to perceive the processes of plant adaptive evolution.
- Transposable Element
- Long Terminal Repeat
- Terminal Inverted Repeat
- Long Terminal Repeat Retrotransposons
- Miniature Inverted Repeat Transposable Element
In the higher plants, only a small percentage of the genome is required for maintenance of life [1, 2], and transposable elements and the sequences derived from them are scattered in the other highly repetitive DNA regions [3–6]. The transposable elements are classified into the class I transposable elements (retrotransposons), which use a transcript as a template and transfer replicatively, and class II transposable elements (transposons), which transfer DNA by the cut-and-paste mechanism. It is believed that class I long terminal repeat (LTR) retrotransposons and class II miniature inverted repeat transposable elements (MITEs) are major components of the plant genome [7–10]. Such transfer factors can be used as the source of mutations for DNA marker development or gene functional analysis; the transposable elements with a high number of copies can become the entry points for PCR during DNA marker development, and the transposable elements that cause gene disruption enable tagging of genes and contribute to functional genome research [11, 12]. However, the sequences of regions of repetitive DNA other than the known transposable elements [13, 14] are still undetermined, while in the region of repetitive DNA regarded as junk DNA, it has been found that the RNA genes that perform epigenetic regulation of gene expression are also scattered [15–17]. Investigation of the unknown factors in repetitive DNA regions is also important as a key to understanding the mechanisms of genome control and phenotypic expression.
The first authors recently reported a new transposon-like gene, named Revolver, in Triticeae [18, 19]. A part of the reiterated sequence (89 bp) specific to the rye genome was cloned by the genome subtraction technique, which deducts the sequence in common with bread wheat from the rye genome . In order to determine the entire structure of this reiterated sequence, the λ FixII genomic library of the rye-inbred line was screened by using 89 bp of the repetitive clone as a probe, and the base sequence of a region of about 21.6 kb was decoded in three lambda clones. As a result, the insertion-type consensus sequence (92% homology) with a full-length sequence of 3,041 bp sandwiched between 20 bp of specific terminal inverted repeat (TIR) sequence was determined and shown to be similar to the class II transposable elements [18, 21–26]. Revolver contains one gene that codes for an open reading frame (ORF) of a deduced 139 amino acid residue that is actively transcribed into mRNA. The sequence of the 20 bp inverted repeat sequence at both ends is different from that of the known transposons represented by hAT, CACTA, and Mutator, and 10 bp of the tandemly repeated sequence is also repeated in the subterminal region.
In a genomic DNA clones of rye that show homology to Revolver, great structural variation in the region ranging from the first exon to the first intron had arisen and four sequences thought to be nonautonomous factors for Revolver were found. Revolver produces 0.7 kb of mRNA and is conserved in Triticeae. A putative TATA box is located at base 221, with a cap site at base 261 and a possible polyadenylation signal AATAAA at base 2918 . On the other hand, the full length of the nonautonomic factor is 2665 to 4269 bp. The nonautonomic factor has a 37-149-bp homologous region upstream from the transcription start site containing a TIR at the 5' end and a 1294-2112-bp region covering from around the second exon to the 3' end on the 3' side. A region of 549-2007 bps located between them (equivalent to the region from the first exon to the first intron) is destroyed. In rye and barley, the nonautonomic factors sharing each end with Revolver are considered to exist within it .
The number of copies in Triticeae was computed by Southern blot analysis and slot blotting techniques by using cDNA of Revolver as the probe. Consequently, extremely high numbers of Revolver (20,000 copies) have been shown to exist in Dasypyrum villosum and Secale sp., as well as in diploid species such as Triticum monococcum, which is the ancestor of bread wheat and tetraploid species such as emmer wheat, with around 10,000 copies. However, the number of copies is extremely low in hexaploid bread wheat. There has been a large quantitative change during the evolution of Triticeae resulting in amplification of Revolver in some species and its disappearance in bread wheat . The considerable quantitative variability of Revolver among the wheat-related species strongly indicates its propagation or differential loss, activity, and diversity in recent evolutionary times.
In this paper, the structural divergence of Revolver in genomic DNA and RNA was analyzed, and length variants of the Revolver family were used as chromosome tags to search the publicly disclosed expressed sequence tag (EST) database of the 440,000 Triticeae EST sequences for Revolver homologues. Moreover, we examined the quantitative variations of Revolver in 161 T. dicoccoides accessions  representing 18 populations collected from various locations in Israel, which encompass a wide range of ecologic conditions of soil, temperature, altitude, and water availability, to determine the effects of ecologic stress on quantitative variation.
Structural diversity of RevolvermRNA
Moreover, cDNAs exhibiting completely different structures at the first exon were found in 50 cDNA clones from self-fertile rye (Figure 1). Among them, five cDNAs had a total length of 1,597 bp and contained the second intron (1,210 bp) and the third exon (301 bp) of Revolver, but at the 5' terminus, they had an 86 bp sequence that was not observed in Revolver. On the other hand, four cDNA clones had a total length of 395 bp and lacked the second intron compared to the cDNA clones of 1,597 bp described above. Meanwhile, another cDNA clone (total length of 2,182 bp) had the second exon (90 bp) and the third exon (260 bp) of Revolver, but the region corresponding to the first exon was extremely long and had no homology with the other cDNA clones. As stated above, the members of the Revolver family, having a common structure downstream of the second intron, may easily undergo various structural changes at the 5' first exon and then become divergent and show different lengths. Such structural diversities were found in the first exon of the Revolver genes that were obtained from the self-fertile pure rye strains, and these structural diversities may be useful for development of DNA markers in the rye genome.
Expressed sequence tag homologues of Revolver
Development of chromosomal markers with Revolver
A typical genomic clone of Revolver (Revolver-2 ) was located in this study on the rye 7R chromosome because 492 bp of DNA was amplified only from the rye 7R chromosome addition line by PCR using the 5'-flanking region of Revolver-2, 5'-GCCTTTCGGCCTTCCTCTCAGGCGG-3', and its internal sequence of 5'-GTACTTGGCATCGGTAGATGTTCGG-3', as the primers. PCR was performed then with the 3'-flanking region derived from the typical genomic clone of Revolver-2 as a single primer, and 4 DNA fragments (2.3 kb, 2.8 kb, 3.3 kb, and 4.3 kb) were amplified from the rye genome, but nothing was amplified from the wheat genome (Figure 4A). Furthermore, when PCR was performed with the same primer and genomic DNA from rye chromosome addition lines as a template, DNA fragments of 2.8 kb, 3.6 kb, and 4.3 kb were amplified from the 1R, 5R, and 6R chromosome addition lines, respectively (Figure 4B). PCR amplification with this single primer identified rye chromosomes 1R and 5R simultaneously. Each DNA fragment derived from the chromosome addition lines and four types of DNAs amplified from the rye genome were sequenced (Additional file 2); and the variants were shown to have the downstream region of the second intron, but they had structural modifications at the 5' first exon region as in the cDNAs (Figure 4B). Such a difference in length in Revolver allows the development of rye chromosome markers.
Revolver-3  comprises a total length of 4,269 bp, and at the 3' end it has a region of 2,112 bp from the middle of the first intron of Revolver through the third exon and reaches the 3'-terminal region (Figure 4B). In this study, Revolver-3 was shown to be localized on the 6R chromosome because it was amplified with the 3'-flanking region primer of Revolver-2 only from the rye 6R chromosome addition line. Furthermore, Revolver-5 located on the rye 1R chromosome had a total length of 2,665 bp, while at the 3' side it had a region of 1,826 bp extending from immediately before the second exon to the 3' terminus of Revolver (Figure 4A and 4B). At its 5' side, the region homologous to Revolver is limited to only 37 bp at the terminus; but a region of about 670 bp is homologous to Revolver-4 , which consists of 3,219 bp, and at the 3' end it has a region of 1,806 bp ranging from immediately before the second exon to the 3' terminus of Revolver (Figure 4B). Finally, Revolver-6 located on the rye 5R chromosome (Figure 4A) had a total length of 3,503 bp, and at the 3' side, it had a region of 1,294 bp from the middle of the second intron to the 3' terminus of Revolver. However, at the 5' side, there was no region homologous to Revolver, and the 121 bp sequence at the 5' terminus was homologous to Revolver-4 and Revolver-5 (Figure 4B).
As mentioned above, the members of the Revolver family showed considerable length variation which was attributed to structural changes in the first exon. Such a divergence in length is also found in some transposons, the CACTA family , the Mutator family , and the MITE family , but no homology was detected between Revolver and any of these. If the Revolver family is a transposable element, these variants are assumed to be nonautonomous elements. Revolver showed a variability that was considerably larger than the others. With PCR primers comprising the sequences flanking the length variants of Revolver scattered in the genome, the chromosome on which each Revolver is located can be determined or tagged, and such PCR primers can be utilized for detection and identification of the chromosomes.
Quantitative variation of Revolverin wild Emmer wheat of Israel
All populations were rich in copy number (Additional file 4 and Figure 5). Most populations had a large variance in copy number from around 1,000 to 30,000, despite the predominantly self-fertilizing nature of this species. The populations in the catchment area of Yehudiyya, Gamla, Tabigha, and Rosh-Pinna, where semi-humid and warm climatic conditions prevail, showed high copy numbers, which were almost over 7,000 per haploid genome and included several genotypes having around 20,000 copies (Figure 5). Several populations had higher numbers than 1,100: Yehudiyya shade or sun, Amirim, Bet Oren, Bat Shelomo, Gamla, Giv'at Koah, and J'aba. In the marginal mesic areas of Amirim, Bet Oren, Bat Shelomo, and Giv'at Koah, the copy numbers were very stable: between 6,000 and 8,000. In contrast, the populations of the marginal steppic areas, such as, Mt. Gerizim, Kokhav-Hashahar, and Gitit, included several genotypes with low copy numbers less than 1,000 (Figure 5). This pattern suggests that high Revolver copy numbers in northern wild emmer populations appear to be associated with biotic stresses, e.g., pathogens and population densities.
The copy numbers in the populations of Yehudiyya, Ammiad, and Tabigha showed a broad range (between 20,000 and less than 1,000) (Figure 5). Some populations displayed gradual variations from low to high (e.g., Ammiad, Yehudiyya sun and Yehudiyya shade, J'aba, Amirim, Bet Oren, Bat Shelomo, and Giv'at Koah). About half of the populations had polymodal distribution. Some populations were extremely bimodal: Gamla (4 genotypes were from 3,000-4,000 and 4 genotypes were from 20,000-30,000) and Gitit (4 genotypes around 500 and 3 genotypes above 12,000) (Figure 5). The different modes of distributions among the populations suggest differences in Revolver's mobility in each population. In barley, the selective pressures under hot, dry desert conditions significantly correlate with increasing copy numbers of retrotransposon BARE-1 [32, 33] and microsatellite sequences . Revolver showed a variance considerably larger than BARE-1. The propagation activity of Revolver also might be responsive to both regional and local environmental conditions and its extensive quantitative diversity in the single species T. dicoccoides as well as among its local populations at microsites, such as, Yehudiyya and Tabigha, suggest the recent mobilization of Revolver by ecological stresses.
Our results show that 6,000 or more copies of Revolver exist in the native line from the area that has much precipitation and an average yearly temperature around 20°C; and there was a line that contained as many as 20,000 copies, as does rye wheat. On the other hand, only a few hundred copies existed in the large majority of the lines in the area where the hot-dry monsoon occurred frequently (85 days per year) and the area where the number of sultry nights reached 80 days per year. The remarkable quantitative differences in Revolver within the same species growing in different ecosystems illustrate a strong amplification activity within the last 10,000 years.
Transposon-like factor Revolver (US granted patent 7351536), which is 3041 bp in length and includes a 20-bp TIR on either end, is dispersed within the genomes of Triticum species. Revolver contains an ORF coding for an amino acid sequence of 139 residues that is conserved among Triticum species. In S. cereale, a 0.7-kb mRNA is actively transcribed from this ORF. Southern blot analysis revealed multiple copies of Revolver within Secale and Dasypyrum species, as well as some copies in diploid species, such as, Einkorn wheat and Aegilops tauschii, and in tetraploid species, such as, Triticum durum. In contrast, copies were not found in the hexaploid species T. aestivum, in which transcribed products were also undetected by northern blot analysis. Through evolution, Revolver has been amplified in several Triticum species while being lost in others. It is therefore a useful gene for the development of DNA markers for Triticeae-related species and for use in T. aestivum breeding.
The novel high-copy Revolver family is transcriptionally active in rye. Some of the transposon-like elements exist in high copy numbers in the genomes of most eukaryotes, but the great majority of them are inactive, and only a small portion of them retain the ability to transpose [35, 36]. Very few transposons have been shown to be transcriptionally active. A copia-like retroelement BARE-1 dispersed in 10% of the barley genome  is transcribed in somatic tissues . Some LTR retrotransposons such as tobacco Tnt1, Tto1, and OARE-1 that are largely inactive, can be transcriptionally activated under conditions of biotic and abiotic stress, including wounding, oxidative stress, and pathogen infection [39, 40]. After stress-induced transcription, the rice LTR retrotransposon Tos17 increased in genomic copy number . In maize, a survey of more than 4 × 105 ESTs identified only 56 retrotransposon cDNAs, supporting the notion that most retrotransposons are inactive . Furthermore, most of these maize sequences are derived from the low-to-middle repetitive LTR retrotransposons and not from the very high copy-number elements that have been responsible for doubling the size of the maize genome in the past 5-6 million years. In humans, only 30-60 L1 elements out of 5 × 105 comprising 45% of the genome are thought to be active . In general, high-copy retrotransposons show low-level activity except for BARE-1. Like BARE-1, highly repetitive Revolver is transcribed.
A transcriptionally active Revolver gene is well conserved among the Triticeae members. The methylated and heterochromatic state of most transposons can cause them to change sequences more rapidly than genes [43, 44]. For example, regulation at any stage of the replication cycle for retrotransposons (transcription, translation, reverse transcription, and integration of cDNA elements) can limit transposition. Furthermore, the paucity of maize retrotransposon-derived ESTs indicates that some epigenetic mechanisms might have been repressing the transcription of a large fraction in the genome [15, 45, 46]. In contrast to these silenced retrotransposons, Revolver is transcriptionally active (Figure 1) and might have retained transcriptional activity during the long evolution of Triticeae. The predicted protein encoded by Revolver subfamily I includes a c2rf9 motif of a kinase domain and may serve as a transcription factor among this family. The considerable variation in Revolver copy numbers among T. dicoccoides indicates their propagation activity during the last 10,000 years of T. dicoccoides evolution. The name of the novel transposon-like gene Revolver means a dynamic factor in constructing genomes through evolution of the Triticeae, associated with ecological stresses, and presumably navigated adaptively by natural selection.
Structural diversities of RevolvermRNA
Revolver cDNAs were obtained from self-fertile rye, and structural analysis of the cDNA clones was performed. The primers for amplification of Revolver cDNA were designed from both ends of a full-length cDNA clone. Total RNA for RT-PCR extracted from seedlings was treated with DNase I. First-strand cDNAs were synthesized by AMV reverse transcriptase (Life Science) with an oligo (dT) primer. Reaction mixtures contained 10 ng of template cDNA, 50 pmoles of each primer (5'-GGCACGAGGGTACGAGTCCGAG-3', 5'-GGCACAACTCATGTAAAAGAGGG-3'), 0.4 mM dNTPs, 1 × LA PCR buffer II, 2.5 mM MgCl2, and 0.5 U of LA Taq polymerase (Takara) in a volume of 25 μL. The PCR reaction program consisted of 30 cycles of 30 sec at 95°C, 30 sec at 63°C, and 1 min at 72°C. The RT-PCR products were purified, ligated to the pGEM-T vector (Promega), and sequenced.
The Revolver ORF in cDNA subfamily I (pSc5) was excised by restriction enzyme Sal I and Nco I and integrated into the plasmid vector pET-32a (+) that had also been digested by Sal I and Nco I. The plasmid was transformed into E. coli BL21 (DE3) cells containing pLysS. The cells were grown in 2YT medium containing carbenicillin and chloramphenicol. The Revolver protein was induced by 1 mM IPTG, and the culture was grown at 20°C overnight. Culture at 37°C resulted in production of an inclusion body. The Revolver protein fused to thioredoxin and his•tag was purified by nickel chelating affinity chromatography followed by POROS CM chromatography. After treatment with Factor Xa protease, the enzyme was subjected to Superdex 75 gel filtration. The protein structure encoded by Revolver was predicted by the Protein Folding Recognition program Robetta (http://robetta.bakerlab.org/).
Expressed sequence tag homologues of a new class of transposon Revolver
In this study, we searched the publicly disclosed EST database of Triticeae for Revolver homologues using a default cutoff expectation value of <e-20. Nucleotide sequences were compared with sequences in the non-redundant GenBank+EMBL+DDBJ databases with BLASTN homology search software . Sequence alignment was determined with the computer program DNASIS Pro version 2.10 (Hitachi, Tokyo, Japan). A nonrooted phylogenetic tree was constructed with MEGA version 4.0.2 . The neighbor-joining method  was conducted with Kimura's 2-parameter distances 
Use of Revolverto generate chromosomal markers
The single primer for amplification of Revolver genomic DNA was designed from the 3'-flanking region of a typical clone of Revolver (Revolver-2). Reaction mixtures contained 10 ng of template genomic DNA, 50 pmoles of single primer (5'-GTAGTCGTCAGGAGTCCTCACCA-3'), 0.4 mM dNTPs, 1 × LA PCR buffer II, 2.5 mM MgCl2, and 0.5 U of LA Taq polymerase (Takara) in a volume of 50 μL. The PCR reaction program consisted of 30 cycles of 30 sec at 95°C, 1 min at 55°C, and 3 min at 72°C. The four types of PCR products (2.3 kb, 2.8 kb, 3.3 kb, and 4.3 kb) amplified from the rye genome and rye chromosome addition wheat genomes were purified, ligated to the pGEM-T vector (Promega), and sequenced.
Diversity of Revolverin wild Emmer wheat of Israel
In this study, we examined the copy number of Revolver by slot blot analysis in 161 T. dicoccoides genotypes representing 18 populations collected from various locations in Israel, which cover a wide range of ecologic conditions of soil, temperature, altitude, water availability, and abiotic stresses. The collections were from 14 locations in Israel, namely, Mt. Hermon, Yehudiyya, Gamla, Rosh-Pinna, Tabigha, Mt. Gilboa, Mt. Gerizim, Gitit, Kokhav-Hashahar, J'aba, Amirim, Bet Oren, Bat Shelomo, and Giv'at Koah.
Total DNAs isolated from 161 T. dicoccoides genotypes were blotted onto Hybond N+ membranes (Amersham). The nonradioactive chemiluminescence method (Gene Images, Amersham) was used for probe labeling, hybridization, and detection of hybridization sites. The Revolver cDNA subfamily I (pSc5, 694 bp), which was conserved in Triticum species, was used as a probe. The membranes were hybridized at 60°C for 30 min in hybridization buffer and then hybridized with a labeled probe at 60°C overnight. The membranes were washed in SSC, 0.1% SDS at 60°C for 15 min, then in 0.1 × SSC, 0.1% SDS for 15 min, followed by incubation for 60 min at room temperature in a 10% (w/v) blocking agent in an antibody wash buffer. The membrane was then incubated in the presence of an anti-fluorescein antibody-alkaline phosphatase (AP) conjugate. The unbound conjugate was removed by three washes in 0.3% (v/v) Tween 20 in an antibody wash buffer at room temperature. Hybridization sites were detected with the CDP-star detection reagent. Decomposition of the stabilized dioxetane was catalyzed by the probe-bound AP, and exposure of the X-ray film by the emitted light was then recorded with a fluoro-image analyzer (FUJIFILM FLA-5000). The copy number was calculated on the basis of the most approximate function, which Image Gauge software computed from control slot blot hybridization to a series of measured amounts of Revolver cDNA (0, 10, 100, 1,000, 5,000, 10,000, 15,000, 20,000, 30, 000, 50,000, and 100,000 copies).
The authors acknowledge the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT) for the Grant-in-Aid for Scientific Research No. 22580005 that supported this work to MT. We also thank Japan Science and Technology Agency (JST) for the Adaptable and Seamless Technology Transfer Program through Target-driven R & D No. 08150094 that supported this work to MT. We thank Alex Beharav for statistical support and the Ancel Teicher Research Foundation of Molecular Evolution and Genetics for financial support to EN.
- Flavell RB, Rimpau JR, Smith DB: Repeated sequence DNA relationships in four cereal genomes. Chromosoma. 1977, 63: 205-222. 10.1007/BF00327450.View ArticleGoogle Scholar
- Grandbastien MA: Retroelements in higher plants. Trends Genet. 1992, 8: 103-108.View ArticlePubMedGoogle Scholar
- Barakat A, Carels N, Bernardi G: The distribution of genes in the genomes of Gramineae. Proc Natl Acad Sci USA. 1997, 94: 6857-6861. 10.1073/pnas.94.13.6857.View ArticlePubMedPubMed CentralGoogle Scholar
- Panstruga R, Buschges R, Piffanelli P, Schulze-Lefert P: A contiguous 60 kb genomic stretch from barley reveals molecular evidence for gene islands in a monocot genome. Nucleic Acids Res. 1998, 26: 1056-1062. 10.1093/nar/26.4.1056.View ArticlePubMedPubMed CentralGoogle Scholar
- SanMiguel P, Bennetzen JL: Evidence that a recent increase in maize genome size was caused by the massive amplification of intergene retrotransposons. Ann Bot. 1998, 82: 37-44.View ArticleGoogle Scholar
- Myers BC, Tingey SV, Morgante M: Abundance, distribution, and transcriptional activity of repetitive elements in the maize genome. Genome Res. 2001, 11: 1660-1676. 10.1101/gr.188201.View ArticleGoogle Scholar
- Bennetzen JL: Transposable element contributions to plant gene and genome evolution. Plant Mol Biol. 2000, 42: 251-269. 10.1023/A:1006344508454.View ArticlePubMedGoogle Scholar
- Vicient CM, Jaaskelainen MJ, Kalendar R, Schulman AH: Active retrotransposons are a common feature of grass genomes. Plant Physiol. 2001, 125: 1283-129. 10.1104/pp.125.3.1283.View ArticlePubMedPubMed CentralGoogle Scholar
- Bureau TE, Ronald PC, Wessler SR: A computer-based systematic survey reveals the predominance of small inverted- repeat elements in wild-type rice genes. Proc Natl Acad Sci USA. 1996, 93: 8524-8529. 10.1073/pnas.93.16.8524.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang X, Jiang N, Feschotte C, Wessler SR: PIF- and Pong-like transposable elements: distribution, evolution and relationship with Tourist-like miniature inverted-repeat transposable elements. Genetics. 2004, 166: 971-986. 10.1534/genetics.166.2.971.View ArticlePubMedPubMed CentralGoogle Scholar
- Kumar A, Hirochika H: Applications of retrotransposons as genetic tools in plant biology. Trends in Plant Sci. 2001, 6: 127-134. 10.1016/S1360-1385(00)01860-4.View ArticleGoogle Scholar
- Lunde CF, Morrow DJ, Roy LM, Walbot V: Progress in maize gene discovery: a project update. Funct Integr Genomics. 2003, 3: 25-32.PubMedGoogle Scholar
- Stein N: Triticeae genomics: advances in sequence analysis of large genome cereal crops. Chromosome Res. 2007, 15: 21-31. 10.1007/s10577-006-1107-9.View ArticlePubMedGoogle Scholar
- Wicker T, Narechania A, Sabot F, Stein J, Vu GTH, Graner A, Ware D, Stein N: Low-pass shotgun sequencing of the barley genome facilitates rapid identification of genes, conserved non-coding sequences and novel repeats. BMC Genomics. 2008, 9: 518-10.1186/1471-2164-9-518.View ArticlePubMedPubMed CentralGoogle Scholar
- Sunker R, Girke T, Zhu JK: Identification and characterization of endogenous small interfering RNAs from rice. Nucleic Acids Res. 2005, 33: 4443-4454. 10.1093/nar/gki758.View ArticleGoogle Scholar
- Feschotte C: Transposable elements and the evolution of regulatory networks. Nat Rev Genet. 2008, 9: 397-405. 10.1038/nrg2337.View ArticlePubMedPubMed CentralGoogle Scholar
- Siomi H, Siomi MC: Interactions between transposable elements and Argonautes have (probably) been shaping the Drosophila genome throughout evolution. Curr Opin Genet Dev. 2008, 18: 181-187. 10.1016/j.gde.2008.01.002.View ArticlePubMedGoogle Scholar
- Tomita M, Shinohara K, Morimoto M: Revolver is a new class of transposon-like gene composing the Triticeae genome. DNA Res. 2008, 15: 49-62. 10.1093/dnares/dsm029.View ArticlePubMedPubMed CentralGoogle Scholar
- Tomita M: Revolver-2: a novel transposon-like element from rye. United States Patent: 7351536B2,. 2008Google Scholar
- Tomita M, Akai K, Morimoto T: Genomic subtraction recovers rye-specific DNA elements enriched in the rye genome. Mol Biotechnol. 2009, 42: 160-167. 10.1007/s12033-009-9151-2.View ArticlePubMedGoogle Scholar
- Flavell AJ, Dunbar E, Anderson R, Pearce SR, Hartley R, Kumar A: Ty1-copia group retrotransposons are ubiquitous and heterogeneous in higher plants. Nucleic Acids Res. 1992, 20: 3639-3644. 10.1093/nar/20.14.3639.View ArticlePubMedPubMed CentralGoogle Scholar
- Kumar A, Bennetzen JL: Plant retrotransposons. Annu Rev Genet. 1999, 33: 479-532. 10.1146/annurev.genet.33.1.479.View ArticlePubMedGoogle Scholar
- Kunze R, Well CF: The hAT and CACTA superfamilies of plant transposons. Mobile DNA II. Edited by: Craig NL, Craigie R, Gellert M, Lambowitz AM. 2002, American Society for Microbiology Press, Washington, DC, 565-610.View ArticleGoogle Scholar
- Bennetzen JL: The Mutator transposable element system of maize. Curr Top Microbiol Immunol. 1996, 204: 195-229.PubMedGoogle Scholar
- Feschotte C, Jiang N, Wessler SR: Plant transposable elements: where genetics meets genomics. Nat Rev Genet. 2002, 3: 329-341.View ArticlePubMedGoogle Scholar
- Feschotte C, Zhang X, Wessler SR: Miniature inverted-repeat transposable elements (MITEs) and their relationship with established DNA transposons. Mobile DNA II. Edited by: Craig NL, Craigie R, Gellert M, Lambowitz, AM. 2002, American Society for Microbiology Press, Washington, DC, 1147-1158.View ArticleGoogle Scholar
- Nevo E, Korol AB, Beiles A, Fahima T: Evolution of wild emmer and wheat improvement. Population genetics, genetic resources, and genome organization of wheat's progenitor, Triticum dicoccoides. 2002, Springer, Berlin, 364-Google Scholar
- Wicker T, Sabot F, Hua-Van A, Bennetzen JF, Capy P, Chalhoub B, Flavell A, Leroy P, Morgante M, Panaud O, Paux E, Sanmiguel P, Schulman AH: A unified classification system for eukaryotic transposable elements. Nat Rev Genet. 2007, 8: 973-982. 10.1038/nrg2165.View ArticlePubMedGoogle Scholar
- Lisch D: Mutator transposons. Trends Plant Sci. 2002, 7: 498-504. 10.1016/S1360-1385(02)02347-6.View ArticlePubMedGoogle Scholar
- Nevo E, Beiles A: Genetic diversity of wild emmer wheat in Israel and Turkey. Theor Appl Genet. 1989, 77: 21-455.View ArticleGoogle Scholar
- Peng J, Ronin YI, Fashima T, Roder MS, Li Y, Nevo E, Korol AB: Domestication quantitative trait loci in Triticum dicoccoides, the progenitor of wheat. Proc Natl Acad Sci USA. 2003, 100: 2489-2494. 10.1073/pnas.252763199.View ArticlePubMedPubMed CentralGoogle Scholar
- Vicient CM, Suoniemi A, Anamthawat-Jonsson K, Tanskanena J, Beharav A, Nevo E, Schulman AH: Retrotransposon BARE-1 and its role in genome evolution in the genus Hordeum. Plant Cell. 1999, 11: 1769-1784.View ArticlePubMedPubMed CentralGoogle Scholar
- Kalendar R, Tanskanen J, Immonen M, Nevo E, Schulman AH: Genome evolution of wild barley (Hordeum spontaneum) by BARE-1 retrotransposon dynamics in response to sharp microclimatic divergence. Proc Natl Acad Sci USA. 2000, 97: 6603-6607. 10.1073/pnas.110587497.View ArticlePubMedPubMed CentralGoogle Scholar
- Nevo E: Evolution of genome-phenome diversity under environmental stress. Proc Natl Acad Sci USA. 2001, 98: 6233-6240. 10.1073/pnas.101109298.View ArticlePubMedPubMed CentralGoogle Scholar
- Grandbastien MA: Activation of plant retrotransposons under stress conditions. Trends Plant Sci. 1998, 3: 181-187. 10.1016/S1360-1385(98)01232-1.View ArticleGoogle Scholar
- Kumar A, Bennetzen JL: Plant retrotransposons. Annu Rev Genet. 1999, 33: 479-532. 10.1146/annurev.genet.33.1.479.View ArticlePubMedGoogle Scholar
- Suoniemi A, Anamthawat-Jonsson K, Arna T, Schulman AH: Retrotransposon BARE-1 is a major, dispersed component of the barley (Hordeum vulgare L.) genome. Plant Mol Biol. 1996, 30: 1321-1329. 10.1007/BF00019563.View ArticlePubMedGoogle Scholar
- Suoniemi A, Narvanto A, Schulman AH: The BARE-1 retrotransposon is transcribed in barley from an LTR promoter active in transient assays. Plant Mol Biol. 1996, 31: 295-306. 10.1007/BF00021791.View ArticlePubMedGoogle Scholar
- Vernhettes S, Grandbastien MA, Casacuberta JM: In vivo characterization of transcriptional regulatory sequences involved in the defence-associated expression of the tobacco retrotransposon Tnt1. Plant Mol Biol. 1997, 35: 673-679. 10.1023/A:1005826605598.View ArticlePubMedGoogle Scholar
- Takeda S, Sugimoto K, Otsuki H, Hirochika H: Transcriptional activation of the tobacco retrotransposon Tto1 by wounding and methyl jasmonate. Plant Mol Biol. 1998, 36: 365-376. 10.1023/A:1005911413528.View ArticlePubMedGoogle Scholar
- Hirochika H: Activation of tobacco retrotransposons during tissue culture. EMBO J. 1993, 12: 2521-2528.PubMedPubMed CentralGoogle Scholar
- Park ET, Kazazian HH: Biology of manmmalian L1 retrotransposons. Annu Rev Genet. 2001, 35: 501-538. 10.1146/annurev.genet.35.102401.091032.View ArticleGoogle Scholar
- Marillonnet S, Wessler SR: Extreme structural heterogeneity among the members of maize. Genetics. 1998, 150: 1245-1246.PubMedPubMed CentralGoogle Scholar
- SanMiguel P, Gaut BS, Tikhonov A, Nakajima Y, Bennetzen JL: The paleontology of intergene retrotransposons of maize. Nature Genet. 1998, 20: 43-45. 10.1038/1695.View ArticlePubMedGoogle Scholar
- Hamilton A, Voinnet O, Chappell L, Baulcombe D: Two classes of short interfering RNA in RNA silencing. EMBO J. 2002, 21: 4671-4679. 10.1093/emboj/cdf464.View ArticlePubMedPubMed CentralGoogle Scholar
- Llave C, Kasschau KD, Rector MA, Carrington JC: Endogenous and silencing-associated small RNAs in plants. Plant Cell. 2002, 14: 1605-1619. 10.1105/tpc.003210.View ArticlePubMedPubMed CentralGoogle Scholar
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman D: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-3402. 10.1093/nar/25.17.3389.View ArticlePubMedPubMed CentralGoogle Scholar
- Kumar S, Tamura K, Nei M: MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics. 2004, 5: 150-163. 10.1093/bib/5.2.150.View ArticlePubMedGoogle Scholar
- Saitou N, Nei M: The neighbor-joining method: A new method for reconstruction phylogenetic trees. Mol Biol Evol. 1987, 4: 406-425.PubMedGoogle Scholar
- Kimura M: A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J Mol Evol. 1980, 16: 111-120. 10.1007/BF01731581.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.