Skip to main content
Figure 3 | BMC Evolutionary Biology

Figure 3

From: A bacterial genome in transition - an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus

Figure 3

Analysis of transpositional activity of the most abundant IS elements of A. asiaticus 5a2. Transposition of IS elements was analyzed with Southern hybridizations using IS element-specific probes and DNA purified from the same A. asiaticus 5a2 culture in November 2006 and July 2009, respectively. DNA was digested with Eco32I (except for ISCaa2, where HindIII was used). Each visible band corresponds to at least one IS element copy on the respective DNA fragment, as the restriction endonucleases do not cut within the IS elements. IS elements are indicated above each hybridization; lanes 1: DNA isolated November 2006; lanes 2: DNA isolated July 2009. The absence of changes in the banding patterns between both time points indicates that no (major) chromosomal rearrangements due to IS element transposition has occurred.

Back to article page