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Figure 4 | BMC Evolutionary Biology

Figure 4

From: A bacterial genome in transition - an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus

Figure 4

Genomic organization of selected A. asiaticus IS elements and their downstream genes. IS elements are shown as dark grey pentagons; downstream genes as light grey pentagons; inverted repeats are represented by vertical black bars. The distance between IS elements and downstream genes is indicated. Reverse transcriptase PCR was used to test for contiguous transcription (Figure 5); the size of the expected PCR products are indicated as horizontal black lines below each locus. Panels (A) to (J) show the organization of IS elements and their downstream genes. Panels (K) and (L) show the genomic organization of genes used for control reactions in the reverse transcriptase PCR experiments. Aasi_1200 and Aasi_1201 are located in opposite direction on different strands and served as negative control; Aasi_0308 and Aasi_0309 representing the groEL/groES operon served as positive control. An asterisk (*) indicates genes which are not drawn to scale (due to their length). Further details on the genomic organization of the analyzed loci and the downstream genes (including locus_tags of the IS elements) are available in Additional file 2, Table S3.

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BMC Ecology and Evolution

ISSN: 2730-7182

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