Molecular characterization and evolution of a gene family encoding male-specific reproductive proteins in the African malaria vector Anopheles gambiae

Table 2 Summary statistics of polymorphisms and neutrality test for AgAcp34A-1, AgAcp34A-2 and AgAcp34A-3 sequence data

Gene	Species/form	N	n	H	Hd	S	π	θ	S	NS	π_s	π_a	D
*AgAcp34A-1*	*A. gambiae*	28	56	12	0.90	11	0.009	0.008	6	6	0.009	0.010	0.510
	*A. gambiae* M	12	24	6	0.80	4	0.005	0.004	3	2	0.008	0.004	1.030
	*A. gambiae* S	16	32	7	0.83	9	0.009	0.007	3	6	0.008	0.009	0.615
	*A. arabiensis*	10	20	2	0.10	1	0.000	0.001	1	0	0.001	0.000	-1.164
	*A. melas*	2	4	1	0.00	0	0.000	0.000	0	0	0.000	0.000	n.a.
	*A. quadriannulatus*	4	8	3	0.61	2	0.002	0.003	1	1	0.003	0.002	-0.448
	*A. merus*	4	8	3	0.71	4	0.006	0.005	2	2	0.012	0.004	0.889
	total	48	96	20	0.89	46	0.029	0.031
*AgAcp34A-2*	*A. gambiae*	30	60	14	0.77	10	0.005	0.007	4	6	0.006	0.004	-0.968
	*A. gambiae* M	13	26	11	0.87	9	0.007	0.008	3	6	0.010	0.006	-0.442
	*A. gambiae* S	17	34	6	0.67	5	0.003	0.004	2	3	0.003	0.003	-0.817
	*A. arabiensis*	16	32	4	0.24	3	0.001	0.003	1	2	0.002	0.001	-1.548
	*A. melas*	6	12	3	0.73	7	0.012	0.008	1	6	0.007	0.013	1.816
	*A. quadriannulatus*	6	12	1	0.00	0	0.000	0.000	0	0	0.000	0.000	n.a.
	*A. merus*	7	14	4	0.65	3	0.003	0.003	2	1	0.012	0.001	-0.030
	total	65	130	21	0.86	20	0.008	0.013
*AgAcp34A-3*	*A. gambiae*	22	44	18	0.95	36	0.029	0.028	11	20	0.044	0.025	0.088
	*A. gambiae* M	10	20	10	0.93	25	0.029	0.024	8	17	0.043	0.024	0.711
	*A. gambiae* S	12	24	10	0.91	26	0.029	0.024	8	13	0.043	0.022	0.766
	*A. arabiensis*	8	16	8	0.88	20	0.017	0.021	9	11	0.033	0.013	-0.641
	*A. melas*	10	20	4	0.44	3	0.002	0.003	2	1	0.008	0.000	-0.693
	*A. quadriannulatus*	6	12	3	0.59	4	0.006	0.005	3	1	0.017	0.002	0.788
	*A. merus*	10	20	7	0.83	5	0.005	0.005	2	3	0.009	0.004	0.140
	total	56	112	38	0.96	58	0.038	0.038

N = n° individuals; n = n° alleles; H = n° haplotypes; S = n° segregating sites; Hd = haplotype diversity; π = nucleotide diversity; at synonymous (π _s ) and (π _a ) nonsynonymous sites; θ = Watterson estimate of theta; S and NS = total number of silent and replacement mutations; D = Tajima D value; p = statistical significance using confidence levels provided by the coalescent (DNAsp 5.0) of Tajima D values (none significant)

ISSN: 2730-7182