Figure 4

Histogram showing the distribution of expected disruptions in open reading frame in 1000 simulations of nucleotide evolution of filovirus VP35-like sequences in the bat genus Myotis. The simulations start from a center of tree (COT) ancestor with an ORF and use the best-fit model of substitution and the observed maximum likelihood tree with branch lengths as parameters. The observed number of disruptions (zero) is shown by a red bar - a value found in only 0.008 of the simulations. Note that the simulations are conservative because they do not account for selective maintenance involving indel mutations (see Figure 3).