Figure 1

Genome-wide distribution of recombination breakpoints arising during different recombination experiments. A linearised MSV genome schematic divided into seventeen approximately equal segments. The light- and dark-grey hatched regions demarcate the regions swapped between the original wild-type viruses during construction of the chimaeric parental viruses (names indicated under each linearised genome). The total number of recombinants with breakpoints within each segment is represented using the bar graphs, where the recombinant viruses were obtained using the MatMPCPVW+VWMPCPMat chimaeric pair (panels A and B), or the MatMPCPLIRVW+VWMPCPLIRMat chimaeric pair (panels C and D) respectively in MSV-sensitive (panels A and C) and MSV-resistant (panels B and D) maize. The red and blue horizontal bars indicate, respectively, the approximate regions of prominent recombination hot-spots and cold-spots identified in wild-type MSV [13]. Genomic features: V2 = movement protein gene; V1 = coat protein gene; SIR = short intergenic region; C1/C2 = replication-associated protein gene; C1 = repA gene; LIR = long intergenic region.