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Figure 1 | BMC Evolutionary Biology

Figure 1

From: Genomic organization and splicing evolution of the doublesex gene, a Drosophila regulator of sexual differentiation, in the dengue and yellow fever mosquito Aedes aegypti

Figure 1

Aeadsx gene. (A) Genomic organization, splicing variants and protein isoforms of dsx in Aedes aegypti. Male-specific and female-specific exons/protein regions are marked in blue and red, respectively. Exons and introns are not shown to scale. Translational start and stop sites and the poly(A) addition sites are marked. Rectangular striped box within exon 2 represents a 63-bp intronic sequence alternatively removed in Aeadsx transcripts of both sexes (see Figure 5B.3 for further details). All transcripts shown in this picture retain the 63-bp intronic sequence, which encodes an in-frame non-conserved 21-aa sequence. Transcripts without the 63-bp are not shown in this picture and in subsequent paper figures. (B) RT-PCR amplification of Aeadsx sex-specific transcripts. Primers used in this amplification are indicated as short red arrows in Figure 1A.

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