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Table 1 Exon-intron junctions of the Aeadsx gene

From: Genomic organization and splicing evolution of the doublesex gene, a Drosophila regulator of sexual differentiation, in the dengue and yellow fever mosquito Aedes aegypti

Aeadsx gene

Exon No.

Exon size(bp)

Splice-donor

Intron size(bp)

Splice-acceptor

N° of Y

1?

---

---

---

---

 

2

518

   A G

274879

N H

12

  

TTGCAG/gtaggtgtgaggcata

 

tctcctctcttttcag/GCAACC

 

3a

45

   P V

43797

G P

10

  

TACCAG/gtgagttcgctgttga

 

tctcttctggtttcag/TTGGCC

 

3b

48

   T D

85670

D E

9

  

GAACAG/gtgcgtacttccttaa

 

tcgtttccaatttcag/ACGACG

 

4

135

   E G

13860

Q A

11

  

ACGAAG/gtatgggggttcttac

 

cttctctgcctcgcag/GTCAAG

 

5a

461

n.c.

208

Q M

5

  

gttgag/gtcattataagcttcc

 

acataatgaccacagt/GCAAAT

 

5b

465

n.c.

10392

Y D

8

  

cgacag/gtactgctattggagg

 

tcaatccctcaaacag/GATACG

 

6

1063

n.c.

22437

n.c.

11

  

acgaag/gtgagtgttctttttt

 

ctcttttcttcaacag/tttcac

 

7

449

n.c.

---

---

---

  

tacacacgtacacattaaaatag

   

Consensus

   AG/gtRagt

 

YYYYYYYYYYYYNYag/G

8,02 +/- 2,15

  

   C

   
  1. The exon/intron/exon boundaries of Aeadsx gene are reported. Exonic coding sequences are shown in uppercase letters, and non-coding regions (n.c.) are in lowercase letters. Encoded aminoacids are reported above the first base of each triplet. R = A or G nucleotide. Y = T or C or nucleotide. N = any nucleotide. The number of pyrimidines (Y) in the 12 bp preceding the 3' splice-acceptor site (NYag) is indicated. The consensus number of pyrimidines (8.02) was derived from the analysis of 4688 Ae. aegypti splice-acceptor sites. The Aeadsx gene intron 5 (13,860 bp) represents the intron preceding the female-specific exon 5a, and its polypyrimidine stretch does not deviate significantly from the consensus. The splice-acceptor site of the short 208-bp intron preceding the female-specific exon 5b is a weak acceptor site, as is the female-specific exon 5b splice-donor site, which deviates significantly from the consensus sequence.