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Figure 3 | BMC Evolutionary Biology

Figure 3

From: Molecular adaptation of a plant-bacterium outer membrane protease towards plague virulence factor Pla

Figure 3

Analysis of expression of Pla-Epo hybrid proteins in E. coli. Expression of the Pla-Epo-hybrid proteins was analyzed by Western blotting of whole cells with a mixture of anti-Pla, anti-Epo, and anti-Pla-loop antisera. The constructs are indicated with their plasmid names (see Table 1). Migration distance of the Pla isoforms are indicated; pre-Pla is the immature form of Pla, α-Pla and γ-Pla are mature Pla proteins with differing conformations, and β-Pla is the mature autocleaved form of Pla.

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