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Table 1 Base substitutions detected in extant and ancient cpDNA sequences of Norway spruce (Picea abies)

From: Population dynamics and genetic changes of Picea abies in the South Carpathians revealed by pollen and ancient DNA analyses

cpDNA region

CK

MD

B

D

TL

Li

LF

K2i

  

Variable position

  

88

107-126

77

78

82

168

196

197

206

154

157

115

133

114

  

Position total

  

424

443 - 462

629

630

634

834

872

873

882

1081

1084

1279

1297

1464

Seq. length

Rel. freq.

Extant haplotypes

Ht1.

C

12A + 5G

A

A

C

G

T

C

G

T

C

T

T

C

1551

0.103

Ht2.

C

11A + 6G

A

A

C

G

T

C

G

T

C

A

T

C

1551

0.259

Ht3.

C

11A + 6G

C

A

C

G

T

C

G

T

C

A

T

C

1551

0.103

Ht4.

C

10A + 6G

A

A

C

G

T

C

G

T

A

A

T

T

1550

0.017

Ht5.

C

13A + 5G

A

A

C

G

T

C

G

T

C

A

T

C

1552

0.034

Ht6.

C

12A + 5G

C

A

C

G

T

C

G

T

C

A

T

C

1551

0.034

Ht7.

C

10A + 6G

A

A

C

G

T

C

G

T

C

A

T

C

1550

0.017

Ht8.

C

12A + 5G

A

A

C

G

T

C

G

T

C

A

T

C

1551

0.379

Ht9.

T

12A + 4G + 3A + G

A

C

T

G

T

A

G

C

C

A

G

C

1554

0.017

Macrofossil haplotypes

Cone

   

C

A

C

G

T

C

G

  

A

T

C

901

 

S 3.

    

A

A

C

         

124

 

S 4.

           

T

A

   

237

 

S 8.

 

  

C

A

C

G

T

C

G

     

575

 

S 9.

 

C

10A + 7GA + 7G

   

G

T

C

G

  

A

T

C

990

 

S 10.

 

  

A

A

C

G

T

A

G

T

C

  

C

1016

 

S 502-S 545

  

C

12A + 5G

            

211

 

Fossil pollen haplotypes

P1.

  

C

10A + 6G

            

212

 

P2-P3.

    

C

A

C

         

124

 

P4-P11.

       

G

T

C

G

     

251

 

P12.

       

G

T

A

G

     

251

 

P13.

       

G

G

A

G

     

251

 

P14-P15.

               

136

 

P16-P17.

 

              

200

 
  1. (√) successful amplification of the fragment with no variation in the sequence; length of concatenated sequences is shown on the right; rows below the name of the fragments indicate the nucleotide position in the single fragments and in the concatenated sequence, respectively; base substitutions in bold were found only in the fossil material; Ht: haplotype; S: seed; P: pollen