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Table 1 Base substitutions detected in extant and ancient cpDNA sequences of Norway spruce (Picea abies)

From: Population dynamics and genetic changes of Picea abies in the South Carpathians revealed by pollen and ancient DNA analyses

cpDNA region CK MD B D TL Li LF K2i   
Variable position    88 107-126 77 78 82 168 196 197 206 154 157 115 133 114   
Position total    424 443 - 462 629 630 634 834 872 873 882 1081 1084 1279 1297 1464 Seq. length Rel. freq.
Extant haplotypes
Ht1. C 12A + 5G A A C G T C G T C T T C 1551 0.103
Ht2. C 11A + 6G A A C G T C G T C A T C 1551 0.259
Ht3. C 11A + 6G C A C G T C G T C A T C 1551 0.103
Ht4. C 10A + 6G A A C G T C G T A A T T 1550 0.017
Ht5. C 13A + 5G A A C G T C G T C A T C 1552 0.034
Ht6. C 12A + 5G C A C G T C G T C A T C 1551 0.034
Ht7. C 10A + 6G A A C G T C G T C A T C 1550 0.017
Ht8. C 12A + 5G A A C G T C G T C A T C 1551 0.379
Ht9. T 12A + 4G + 3A + G A C T G T A G C C A G C 1554 0.017
Macrofossil haplotypes
Cone     C A C G T C G    A T C 901  
S 3.      A A C           124  
S 4.             T A     237  
S 8.     C A C G T C G       575  
S 9.   C 10A + 7GA + 7G     G T C G    A T C 990  
S 10.     A A C G T A G T C    C 1016  
S 502-S 545    C 12A + 5G              211  
Fossil pollen haplotypes
P1.    C 10A + 6G              212  
P2-P3.      C A C           124  
P4-P11.         G T C G       251  
P12.         G T A G       251  
P13.         G G A G       251  
P14-P15.                 136  
P16-P17.                 200  
  1. (√) successful amplification of the fragment with no variation in the sequence; length of concatenated sequences is shown on the right; rows below the name of the fragments indicate the nucleotide position in the single fragments and in the concatenated sequence, respectively; base substitutions in bold were found only in the fossil material; Ht: haplotype; S: seed; P: pollen