Figure 4

Tissue expression of the chicken PTH1R and PTH3R in adult (A) and embryonic stages (B). The RT-PCR reactions for A) and B) were carried out simultaneously using transcript specific primers and ribosomal subunit 18 S as reference. In B the chicken the embryos were at different Hamburger and Hamilton stages, (HH) and CAM was obtained from stage 44HH. B-brain (forebrain, midbrain, hindbrain); I-intestine; Dden-duodenum; Mid-midgut; Hind-hindgut; Pth-parathyroid; Lu-lung; K-kidney; Li-liver; M-muscle; Bn-bone; C-cartilage; P-pituitary; CAM- chorionallantois membrane; -ve-negative.