Figure 4

Multi-species AKAP7 gene alignments. (A) AKAP7 gene sequence and coding regions of AKAP7 long form splice variants δ and γ from several mammals were aligned using Clustal. Translated protein sequence has been added for splice variants with EST or sequencing support from this study. Position of the AKAP7δ 5’ exon and flanking intron are indicated with solid lines and arrows, the 5’ and 3’ splice junctions are denoted with (҂). The AKAP7δ 5’ exon is only present in the AKAP7 genes of cow, rat, mouse, and primates. The putative AKAP7γ start codon is indicated with (*), the methionine (M) at this position has mutated to a glycine (G) in rats, valine (V) in mice, and isoleucine (I) in marmoset. AKAP7γ is initiated at a downstream methionine in mice, indicated by (#). This codon is mutated to a leucine (L) in rats. Species where AKAP7δ is the only AKAP7 long form splice variant produced are indicated by (**). (B) A protein model was constructed to represent the AKAP7 long form splice variant complement for species shown in (A). The ancestral AKAP7γ splice variant is present in cow and human, absent in rat, and truncated in mouse.