Figure 7

Insertion-Deletion Variants of Human AKAP7δ. (A) PCR amplification of human AKAP7δ shows two splice variants of different length following agarose gel electrophoresis. (B) In AKAP7δ variant-1 an insert sequence is found between AKAP7δ exons-1 and 2 and AKAP7δ exon-6 is deleted. An insert sequence between exons-6 and 7 is identified in AKAP7δ variant-2 in addition to the 5’ insert sequence. (C) PCR amplification was used to determine the expression of AKAP7δ variants-1 and 2 in human tissues (relative docking sites for primer pairs are shown in 7B). In PCR set I, the 5’ insert sequence is predominantly present in all tissues relative to the non-insert product (native). In PCR set II, the native form is the major product relative to the AKAP7δ exon-6 deletion found in variant-1. Shown in PCR set III, the 3’ insert sequence found in variant-2 is a minor product in the liver and pancreas relative to the native form.