Evolution of specifier proteins in glucosinolate-containing plants

Table 2 Activity of newly identified specifier proteins

	Incubation with myrosinase and allylglucosinolate			Incubation with myrosinase and benzylglucosinolate
	Thiocyanate	Epithionitrile	Simple nitrile	Thiocyanate	Simple nitrile
ApTFP1	+	+	-	-	+
ChESP1	-	+	-	-	+
CiESP1	-	+	-	-	+
DaESP1	-	+	-	-	+
DlESP1	-	+	-	-	+
ItESP1	-	+	-	-	+
SpESP1	-	+	-	-	+
ChNSP1	-	-	+	-	+
ItNSP1	-	-	+	-	+
SpNSP1	-	-	+	-	+

Activity was tested using crude extracts of bacteria expressing specifier protein cDNAs, 1 mM allyl- or 2 mM benzylglucosinolate and 0.005 units myrosinase in 50 mM MES buffer, pH 6.0. The presence of non-isothiocyanate products (organic thiocyante, epithionitrile, simple nitrile) in dichloromethane extracts of the assay mixtures after 30 min incubation is indicated by +, the absence by - (in comparison to assays with extracts of bacteria harbouring the expression vector without cDNA insert, for chromatograms see Additional file 5: Figure S3).

ISSN: 2730-7182