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Table 2 Activity of newly identified specifier proteins

From: Evolution of specifier proteins in glucosinolate-containing plants

  Incubation with myrosinase and allylglucosinolate Incubation with myrosinase and benzylglucosinolate
  Thiocyanate Epithionitrile Simple nitrile Thiocyanate Simple nitrile
ApTFP1 + + - - +
ChESP1 - + - - +
CiESP1 - + - - +
DaESP1 - + - - +
DlESP1 - + - - +
ItESP1 - + - - +
SpESP1 - + - - +
ChNSP1 - - + - +
ItNSP1 - - + - +
SpNSP1 - - + - +
  1. Activity was tested using crude extracts of bacteria expressing specifier protein cDNAs, 1 mM allyl- or 2 mM benzylglucosinolate and 0.005 units myrosinase in 50 mM MES buffer, pH 6.0. The presence of non-isothiocyanate products (organic thiocyante, epithionitrile, simple nitrile) in dichloromethane extracts of the assay mixtures after 30 min incubation is indicated by +, the absence by - (in comparison to assays with extracts of bacteria harbouring the expression vector without cDNA insert, for chromatograms see Additional file 5: Figure S3).