- Research article
- Open Access
Reconstructing the history of a fragmented and heavily exploited red deer population using ancient and contemporary DNA
BMC Evolutionary Biology volume 12, Article number: 191 (2012)
Red deer (Cervus elaphus) have been an important human resource for millennia, experiencing intensive human influence through habitat alterations, hunting and translocation of animals. In this study we investigate a time series of ancient and contemporary DNA from Norwegian red deer spanning about 7,000 years. Our main aim was to investigate how increasing agricultural land use, hunting pressure and possibly human mediated translocation of animals have affected the genetic diversity on a long-term scale.
We obtained mtDNA (D-loop) sequences from 73 ancient specimens. These show higher genetic diversity in ancient compared to extant samples, with the highest diversity preceding the onset of agricultural intensification in the Early Iron Age. Using standard diversity indices, Bayesian skyline plot and approximate Bayesian computation, we detected a population reduction which was more prolonged than, but not as severe as, historic documents indicate. There are signs of substantial changes in haplotype frequencies primarily due to loss of haplotypes through genetic drift. There is no indication of human mediated translocations into the Norwegian population. All the Norwegian sequences show a western European origin, from which the Norwegian lineage diverged approximately 15,000 years ago.
Our results provide direct insight into the effects of increasing habitat fragmentation and human hunting pressure on genetic diversity and structure of red deer populations. They also shed light on the northward post-glacial colonisation process of red deer in Europe and suggest increased precision in inferring past demographic events when including both ancient and contemporary DNA.
Humans have a major impact on the evolution and survival of other life forms . Our activities may modify the genetic diversity and structure of other species through e.g. heavy harvesting, habitat fragmentation and translocation of populations [2, 3], often reducing their fitness and future adaptive potential [4, 5]. Red deer (Cervus elaphus) are one of the most common and widespread of European ungulates today . However, due to heavy hunting and habitat alterations, many populations were severely reduced in numbers in previous centuries. Several populations were driven to extinction or confined to scattered and isolated refugia from which they have later expanded . Even today the widespread distribution is often patchy and fragmented, a trend that is increasing in some areas as a result of habitat loss and overhunting . Red deer have also been subjected to poorly documented human mediated translocations of animals, often over large distances, leading to mixing with or substitution of the indigenous population . Such translocations could potentially disrupt long-term local adaptations of populations .
Norwegian red deer represents the end point of the northward post-glacial colonisation of the western European red deer lineage  and relatively low genetic diversity has been found in contemporary populations [10, 11]. Traditionally it was described as a separate subspecies (C. e. atlanticus), but this is not supported in more recent analyses based on skull morphology  or molecular phylogenetics [13, 14]. Norwegian deer are, nevertheless, differentiated both morphologically and genetically from Swedish and Danish populations and seem more closely related to Scottish red deer [10, 15]. This differentiation among Scandinavian red deer has been explained either by post-glacial separation and adaptations to different environments, or alternatively, that they originate from different source populations [15, 16]. Explanations for the latter hypothesis suggest two waves of post-glacial immigration to northern Europe, with the first wave dominating the Norwegian population, and a later human mediated translocation of animals possibly by Vikings [10, 15, 17]. There are numerous findings of prehistoric red deer bones in Norway and, according to older documents e.g. , they were widespread and numerous in the 16th century AD before rapidly declining in numbers, allegedly as an effect of increased hunting pressure and wolf predation. A similar decline was reported in Sweden  and in the 19th century the Scandinavian red deer had been reduced to a few isolated locations, estimated to consist of only a few hundred animals in total . Following strict hunting regulations, an almost exponential growth took place during the last century and the current estimated Norwegian census size is well above 100,000 individuals, mainly distributed along the west coast of the Scandes mountains .
In this study we analyse contemporary and ancient genetic diversity in Norwegian red deer in a time-series of mitochondrial DNA (mtDNA) sequences spanning the last 7,000 years. Our aim is to estimate the timing, pattern and magnitude of the historic population decline using different methods to detect past demographic changes. In addition, we explore the relationship to other European populations and the possibility of human translocation of red deer into Norway. We analyse contemporary DNA together with ancient DNA (aDNA) using Bayesian methods and investigate whether including aDNA significantly changes the outcome of our analyses. Our results provide direct insights into the effects of increasing agricultural land use and human hunting pressure on genetic diversity and structure on a long timescale, and shed light on the northward post-glacial colonization process of red deer in Europe.
Sampling and dating
A total of 142 Holocene samples of subfossil red deer skeletal remains were collected from nine archaeological sites in Norway for aDNA analysis of the mtDNA control region (D-loop) (Figure 1 and Table 1). Samples were 14C dated when originating from poorly dated sites or with an insecure stratigraphy (Additional file 1: Table S1). 14C dates were calibrated using CALIB 5.0.1  with Incal04 calibration curve  and all dates reported in the text are in calendar years before present (yr BP). The age of the samples range from the Mesolithic to the Late Middle Ages (c. 7,000-500 yr BP) and all samples were collected from the University Museum of Bergen. Data on contemporary Norwegian and European mtDNA diversity were compiled from the literature ([14, 23–29], Haanes et al. unpublished results), excluding sequences from populations described by authors as translocated by humans during the last centuries. Nine new sequences from contemporary Norwegian animals were also included (see Additional file 1: Table S2 for overview of contemporary samples).
DNA extraction, amplification and sequencing
We drilled out 30–50 mg of powder from the samples using a Dremel multitool on slow speed. DNA was extracted using Qiagen DNeasy Tissue Kit by adding to the samples 300 μl Buffer ATL, 200 μl 1 M EDTA (pH 8) and 35 μl proteinase K, incubating in a thermomixer at 55°C over night. We used 450 μl of this in extractions following the Qiagen protocol. Polymerase chain reaction (PCR) amplifications of a 327 bp long sequence were performed using two pairs of overlapping primers which were first tested using contemporary samples of red deer:
(Ce116F) 5Â´-CCACCAACCACACAACAAAA-3Â´ with (Ce343R) 5Â´-GATCTAGGGGACGGGATACG-3Â´;
(CeCRF251) 5Â´-TGCCCCATGCATATAAGCATG-3Â´ with (CeCRR519) 5Â´-TAGGTGAGATGGCCCTGAAAAAAG-3Â´.
Amplifications were performed in 25 μl reaction mixtures containing 4–8 μl DNA extract, 0.625 U of PfuTurbo Hotstart DNA Polymerase (Stratagene), 2.5 μl 10x Pfu buffer, 12.5 pmol of each primer, 2.5 μg bovine serum albumin (Sigma) and 200 μM of each dNTP. The PCR profile was 2 min denaturation at 95°C followed by 46 cycles of 30 s denaturation at 95°C, 30 s annealing at 72°C, 60 s of extension at 72°C, and a final extension step of 10 min at 72°C. Amplified PCR products were cleaned using ExoSAP-IT (USB). Sequencing of both strands was performed using BigDye terminator cycle sequencing kit v.1.1 on an ABI 3100 genetic analyser. Sequences were inspected and aligned by eye with aid of MEGA v.4 .
Several precautions were taken to ensure amplification of authentic DNA from the ancient samples . All equipment and working surfaces were cleaned using sodium hypochlorite, ethanol or UV-light. Drilling and extraction were done in designated labs physically separated from post-PCR laboratories, and where no previous work on red deer had been done before. Lab coats and breathing masks were used, gloves were changed frequently and drill bits were changed for each sample. Samples were mechanically cleaned and the outer surface was removed before drilling out the powder. Blank extraction and PCR controls were used in each reaction and only DNA sequences which could be replicated from at least two independent amplifications of each primer pair were used in the subsequent analyses. In addition, the primer pairs were overlapping for 84 bp of the target sequence yielding four replicates or more of the most variable region, thereby ensuring that nuclear copies (NUMTS) could be avoided . We cloned and sequenced a sample of each new haplotype found, using 10 clones from each sample. Cloning was performed according to the Topo TA Cloning protocols (Invitrogen) with PCR-products extracted from gel using the MinElute Gel Extraction Kit protocol (Invitrogen).
Analysing genetic diversity and population structure
A previous analysis using microsatellites found a strong genetic differentiation of the present Norwegian populations north and south of the Sognefjord area . We therefore pooled our contemporary data into three geographic regions along a north–south transect: the northern part of the distribution, including Møre & Romsdal and Trøndelag counties (N); the Sognefjord area (W); and the southwest, including Rogaland and Hordaland counties (SW). We tested for population structure between the three areas by calculating F-statistics using only haplotype frequencies (FST)  and ΦST which also accounts for genetic distance between haplotypes . Significance values were obtained after 10,000 permutations using the software Arlequin v. 3.1 .
Estimates may become biased when analysing populations with a pronounced population structure [37–49], and to better meet assumptions of panmixia we excluded “site 1” from the ancient samples and the N region from the contemporary data set focusing only on “western Norway” on subsequent analyses of genetic diversity through time. The data was divided into four time periods which were treated as separate “populations” in the analyses: c. 7,000-3,500 yr BP (mid-Holocene); c. 2,500-2,000 yr BP (representing the period before any suspected bottleneck and translocations); c. 1,500-500 yr BP (a period including the human Migration Period, the Viking Age and the Middle Ages); and the present. We calculated standard genetic diversity indices, θk, Tajima’s D  and Fu’s Fs on both the present and ancient data sets using the software Arlequin.
Bayesian skyline plot
A Bayesian Skyline Plot (BSP)  implemented in BEAST v.1.6.1  was used to explore past demographic changes in the red deer population. This approach was also used to obtain a direct estimate of substitution rate by using the age of individual sequences as calibrating information . Sequence ages were rounded to the nearest 500 yr BP as most samples were not directly dated but were obtained from well dated stratigraphic layers with a certain age range (Additional file 1: Table S3). Substitution model was selected using the Aikake information criterion in jModelTest v. 0.1.1 [46, 47], suggesting that the HKY model had the best fit to the data set . Both a strict and a relaxed molecular clock were applied; the results were similar and a strict clock was used for the final runs. We summarized the lineage coalescent events into ten groups to smooth the estimates over time. MCMC was run for 100 mill iterations, sampling every 10,000th step and with a 10% burn-in. To estimate model parameters we ran two independent runs which were combined and inspected using TRACER v.1.5 . To evaluate support for the BSP model we also did a run using a constant size coalescent tree prior instead of the BSP, comparing the different runs using Bayes factors .
Approximate Bayesian computation analysis
We estimated historical parameters for the Norwegian red deer; i.e. time since immigration(s), effective population size through time, and the genetic effect of bottleneck(s) in modern times; in a coalescence framework using approximate Bayesian computation (ABC)  and the DIY-ABC software ver. 126.96.36.199 . Based on the results from the above analyses we explored two different scenarios with either one (scenario 1) or two colonisation events (scenario 2), with the second being a late translocation event possibly from the British Isles during the Viking ages (Figure 2).
In two initial runs we compared the effects of using larger effective population size of females than of males (with a ratio of 0.1), compared to a scenario where they were equal, using similar parameter settings and prior ranges as described below. The results did not differ between the two approaches (results not shown), and we therefore did a full scale analysis assuming an equal sex ratio. We modelled two populations: Norway and Europe, with current effective population sizes NNOR and NEUR respectively. To represent the European diversity, one haplotype from 15 different western European populations were randomly picked, 13 from Skog et al. , one from Nielsen et al.  and one from McDevitt et al. . Such sampling should yield properties approximating those of a random mating population [53, 54]. We used the same time periods as described above to represent a serial sampling of the population at four time points (t0-t3), with t0 representing the contemporary sample and t3 the oldest of the ancient samples (Figure 2). In order to convert time estimates to years instead of generations, we assumed a generation time of four years in female red deer . In scenario 1, the Norwegian population went through a population bottleneck starting at tBS and ending at tBE years back in time, where the effective population size of the Norwegian population is NB. At time t1, which we allow to occur either within or before the bottleneck period (i.e. we do not a priori determine the relative size of tBS and t1), the Norwegian population is sampled, and prior range for t1 is set to between 500 and 1,700 yr BP. The sampling at year t2 and year t3 is assumed to happen before tBS, i.e., in a period with effective size NNOR, with prior ranges 2,000 – 2,500 yr BP for t2 and 3,500 – 7,000 yr BP for t3. In the period between tD – x and tD the Norwegian population experienced a founder event as a result of the colonisation of Norway, with tD marking the time of divergence from the European population. In scenario 2 we assumed a similar history as in scenario 1, except that there was an immigration and admixture event from the European population before tBS and either before or after the sampling at t2. The second immigrated population underwent a bottleneck for a total of x years and has an effective size NNOR at the time of admixture. A fraction r of the original Norwegian population and a fraction 1 – r of the second immigration contribute to the admixed present day population.
We assumed no migration between populations after divergence, implying that the divergence time estimates are to be treated as minimum estimates. Prior ranges of the effective population size were set to 1,000 – 100,000 for NNOR and NB, and 1,000 – 1,000,000 for NEUR. Based on the results from the Bayesian skyline plot (see below) we assumed that a putative bottleneck started between 500 – 2,500 yr BP and ended 1 – 500 years ago. The postglacial divergence of the Norwegian and European populations was set to occur between 9,000 and 20,000 yr BP. In scenario 2 the second divergence of the Norwegian population from the European was set to occur between 3,500 and 20,000 yr BP, with an original bottleneck lasting between 1 and 500 years. The admixture between the first and the second Norwegian populations occurred between 500 and 2,500 yr BP, before or after the sampling at t2.
Mutation rates were treated as nuisance parameters, and we assumed a Kimura 2-parameter model  with prior mutation rate estimate range obtained from the BEAST analyses (see below). Summary statistics were computed for each sample in each simulation, i.e. number of haplotypes, Tajima’s D, number of private segregating sites and average number of pairwise differences for each pair of populations sampled together. Each scenario was tested using 1 mill simulations, i.e., 2 mill simulations in total for the testing of scenarios 1 and 2.
The scenarios were compared using two approaches; one by directly comparing the summary statistics with the observed diversity in the data set and counting the frequency of the various scenarios among the most similar simulated datasets [56, 57], and one by doing a logistic regression of each scenario probability for the most similar simulated data sets on the deviations between simulated and observed summary statistics [58, 59]. In the direct comparisons approach the 500 simulated data closest to the observed values were used, while in the regression approach we used 1% of the simulated data closest to the observed data set. Confidence in scenario choice was evaluated by choosing scenario 1 as the true scenario and then simulating 500 data sets using this scenario and parameter values drawn randomly from the prior distributions, and then doing the same for scenario 2. The proportion of times the most likely scenario did not have the highest posterior probability when it was the true scenario was used as an estimate of type I error. The number of times the most likely scenario had the highest probability when it was not the true scenario was used as an estimate of type II error. Parameters were estimated for the most likely scenario using the 1% simulations for a given scenario most similar to the observed data set for the summary statistics employed. In order to evaluate the performance of the estimation procedure, we generated pseudo-observed data sets with known parameter values drawn from the posterior distribution given the most likely scenario. The mean relative bias (MRB), , was estimated, where e i is the i’th estimate of the pseudo-generated true value v, and averaged over the n = 500 data sets.
In order to study the impact of including aDNA samples, we chose the scenario with the highest estimated posterior probability (scenario 1, see below), and re-ran the ABC analyses using only extant DNA samples. In these simulations we assumed a similar history as in scenario 1 above, except that we had no aDNA samples, i.e. t1 – t3 were not included.
Relationships between haplotypes
Relationships between haplotypes were investigated both on a national (Norwegian) and western European level using three different phylogenetic network methods: median-joining in Network v.188.8.131.52 , statistical parsimony in TCS v.1.21  and NeighborNet in SplitsTree4 v.4.11.3 .
Amplification success and authentication of ancient samples
We successfully sequenced 73 (51.4%) of the ancient samples from which we identified 10 haplotypes: NO1 – NO10 [GenBank: JX861260-JX861269] (Table 1; Additional file 1: Table S3). A BLAST search  revealed that three haplotypes (NO8 – NO10) were novel. The three network methods yielded the same relationship between the ten Norwegian haplotypes showing a close relationship and a star-like pattern with NO4 as the central type (Figure 3).
None of the negative controls contained red deer DNA, but some samples showed contamination from cattle (Bos taurus) when using primer pair II, a common problem in aDNA studies . All clones produced identical sequences, except four clones in sample A1348 (NO9) which showed a G-A transition, typical of post-mortem DNA decay . This was only expressed in the clones and was distinct from the segregating site that distinguished this haplotype. Most of the variable sites were also within the overlapping part of the two primer pairs and the independent replicates were always identical. We therefore have good reason to believe that all resulting sequences are authentic.
Present population structure and diversity
176 control region sequences (nN = 86; nW = 45; nSW = 45) were gathered from the extant Norwegian red deer distribution. From these we identified five different haplotypes (NO1 – NO5), identical to five of the ancient samples. The frequency and distribution of the haplotypes differed strongly among the three regions (Figure 1), with a significant genetic structure (global ΦST = 0.663, P < 0.001; global FST = 0.528, P < 0.001). Pairwise population differentiation was very high between regions and the most geographically distant populations were also the most genetically differentiated (Table 2). Overall haplotype diversity was moderate in the extant population while nucleotide diversity and θk were low, both seemingly lowest in the northernmost area (Table 2). Among the Tajima’s D or Fu’s Fs values only the D-value of the Sognefjord population (W) and the combined total genetic diversity of Norwegian deer differed significantly from equilibrium expectations, indicating a recent bottleneck.
Genetic diversity through time in western Norway
Considering only western Norway from where we have most of our ancient samples (70/73) it is clear that both the genetic diversity (Table 3) and the relative frequencies of haplotypes (Figure 3) have changed through time. The Early Iron Age samples (about 2,500-2,000 yr BP) show the highest diversity indices, while the number of haplotypes seems to have been reduced to the five types (NO1-NO5) found in the contemporary population already before the Middle Ages. Further loss of diversity is apparent when proceeding to the present as the once dominant haplotype NO4 has been lost in western Norway (W and SW areas) and is only found in the northern area in the present samples. θk-values are higher in all the ancient periods compared to the present (Table 3), indicating larger effective female population size in the past. None of the Tajima’s D or Fu’s Fs values are significant except for the negative Fs value of the mid-Holocene samples (in bold), which remains significant even after Bonferroni correction, indicating population expansion. Among the five haplotypes found in the extant population all but NO3 have been found in samples older than any suspected translocation events.
Bayesian skyline analysis
The BSP suggest a relatively stable female effective population size, although with a wide confidence interval, until about 2,000 yr BP, when effective size starts to decrease in a stepwise manner (Figure 4). A Bayes factor of 106.8 indicates a strong support for the BSP model over the constant size model. The estimated substitution rate was 2.78 ×10-7 (95% highest posterior density interval (HPDI): 8.15 ×10-8 – 5.23 ×10-7) substitutions per site per year, which is in line with other studies using aDNA datasets [66, 67] and the rate estimated for red deer using only present European diversity .
Both in the direct and logistic approach, scenario 1 in Figure 2 has the highest support (Additional file 1: Figure S1), i.e. a single colonisation of Norway. The estimated type I and type II error rates are 13% and 21% respectively, implying a statistical power of 79%. The estimated historical parameters based on scenario 1 are presented in Table 4. Median effective sizes of female Norwegian and European populations are NNOR = 7,160 (95% credible interval 1,870 – 32,000) and NEUR = 1.03 mill (95% CI 325,000 – 3.2 mill) and the European and Norwegian populations seemingly diverged from one another tD = 15,280 (95% CI 9,960 – 19,560) years ago. There are only weak signs of bottleneck as modal values of NB = 10 (95% CI 1,110 – 85,700), but median value being NB = 22,700, i.e., larger than the NNOR estimate. The larger median value of NB compared to NNOR is likely an effect of assuming an equal population size before and after the bottleneck. The median time with varying population sizes is tBS – tBE = 976 years (95% CI 452 – 1,704). All time estimates have relatively flat posterior probability distributions and estimates must be interpreted with caution. The bias (MRB) is low (<1) for NNOR, NEUR and NB effective size estimates and the various time estimates, while moderate for estimated time with possible changes in effective sizes after divergence (x, MRB equals 1.60) (Table 4).
The results from the simulations only using extant samples of DNA, and assuming scenario 1, are shown in parentheses in Table 4. The precision of the estimates are markedly reduced for some of the parameters causing MRB estimates to increase up to 6.8 times for various parameters when excluding aDNA samples compared to estimates including aDNA (average increase in MRB equal to 3.14 across all comparable parameters). However, the parameter estimates have partly overlapping credible intervals and in general the estimates are very similar, except that credible intervals on average is 20% larger in analyses excluding aDNA, i.e., including aDNA is seemingly increasing the precision of estimates.
Placement in the western European lineage
We sampled 131 sequences from the western European lineage which resulted in 83 haplotypes of the analysed fragment (Additional file 1: Table S2). The median-joining and statistical parsimony methods produced similar haplotype networks with the exception of an additional loop in the median-joining (Figure 5). The haplotypes cluster in star-like patterns around two central haplotypes identical to NO1 and NO4, with the central haplotype considered to be NO1 by the TCS program. This dual clustering is also apparent in the NeighborNet (Additional file 1: Figure S2). Five of the Norwegian haplotypes (NO1, NO4 – NO7) are shared with other countries while the other five have so far only been found in Norway.
Red deer colonised western Norway some time before 8,000 yr BP and skeletal remains indicate that they were well established by the start of the period covered by this analysis (Rosvold et al. unpublished results). While significant Fu’s Fs indicate a population expansion during the early part of our study period (Table 3), the BSP (Figure 4) suggests a relatively stable female effective population size until about 2,000 yr BP. However, as the samples for this early period are widely dispersed in time, the posterior density interval of the BSP is large and the estimate is thus insecure. The Early Iron Age sample (c. 2,500 – 2,000 yr BP) has the highest diversity indices of all periods considered (Table 3), indicating that the population size may have been particularly high during this time.
Historical texts tell of a widespread and large population in Norway until around 500 yr BP, after which there was a dramatic decrease in numbers, allegedly caused by high harvesting rates and increased numbers of predators [18, 68]. However, as indicated by the diversity indices, the BSP and the ABC analyses, this decrease appears to have been more prolonged, probably starting before medieval times. The estimated effective population sizes should be interpreted with caution [69, 70], but the relative changes give a reliable picture of the magnitude of the demographic bottleneck. Accordingly, and supported by an earlier study on contemporary microsatellite DNA , the bottleneck seems not to have been as dramatic as the historic texts may suggest. The mtDNA diversity in the present population is, however, relatively low (Table 2) and especially the nucleotide diversity is low compared to other European populations . The lowest diversity estimates are found in the northernmost region (N), as expected from a peripheral population loosing diversity during postglacial colonisation . The current high population density of red deer in Norway is generally believed to be a recent phenomenon , but our results might indicate that at least the effective female population size, as measured through genetic diversity, was even higher in the past.
Following the spread of agriculture along the coast of western Norway the once dense coastal forests were transformed into the present day heathlands [73, 74]. A process which was greatly accelerated from around 2,000 yr BP through intensified agricultural activities , iron, coal, and salt production, and later by mining and timber export . These changes in the landscape probably facilitated and exacerbated the effects of heavy hunting by increasing habitat fragmentation and possibly reducing migration between areas, thereby isolating populations. The loss of the previously most abundant haplotype (NO4) may indicate extensive genetic drift within these fragmented populations, reducing genetic diversity on a local scale. However, the number of isolated populations were relatively large (at least six) and evenly spread along large parts of its former distribution . Thus, the overall genetic diversity may have been better maintained by the wide geographic spread of the populations than if they had been reduced to a single but larger population . Population fragmentation and isolation is expected to cause increased genetic differentiation , and indeed, there is a high degree of genetic structuring among the present Norwegian female red deer (Figure 1 and Table 2). This indicates that few females have migrated between the areas since the population size reduction, supporting findings that fjords may act as significant dispersal barriers [34, 78].
The ten Norwegian haplotypes observed in the ancient samples are closely related to the rest of the western European clade. The star-like structuring (Figure 3) coupled with low nucleotide diversity (Table 3) is indicative of a population expansion from an ancestral haplotype  which in this case seems to be NO4. This close relationship, coupled with the observation that all present-day haplotypes except NO3 have been found in samples dating to 2,000 yr BP or older, is an indication of no human translocation of female red deer into Norway during historic times. This conclusion is also supported by ABC analyses, where a scenario of only one post-glacial colonisation of Norway gets highest support. NO3 is first found at low frequency in late medieval samples and has so far only been found in Norway (Figure 5). It is possible that NO3 originated in Norway and became frequent in the Sognefjord area (W, Figure 1) as an effect of genetic drift and subsequent population increase during recent times.
An estimated divergence time of the Norwegian population of around 15,000 yr BP (9,960 – 19,560), as indicated by the ABC analysis (Table 4), coincides with the start of the northward colonisation of Europe after the Last Glacial Maximum . The haplotype network for the western European red deer (Figure 5) confirms previous findings of a close relationship within the western European clade [13, 14], with little or no apparent geographic structure and several cross-links indicating uncertain relationships. Out of the ten haplotypes found in the ancient Norwegian dataset five are shared with other countries. Of these, the two central haplotypes NO1 and NO4 are widespread, being present in Scotland and the border forests between Germany and the Czech Republic [14, 23, 25], with NO4 also found in Spain , and NO1 being one of the most common types found in the Scottish highlands today . None of the extant Norwegian haplotypes are shared with other Scandinavian countries; although the ancient NO6 is found in Denmark today . The Swedish population seems to have experienced a more severe bottleneck than the Norwegian as only one haplotype, closely related to NO1, is found among indigenous animals [11, 14]. This low diversity makes it hard to postulate the relationship to Swedish animals, but present Scandinavian diversity indicates that some haplotypes never reached Norway and that a large part of those passing through Denmark during the post-glacial colonization (i.e. the Norwegian types) were later lost. Sampling aDNA from both Sweden and Denmark could shed more light on if this was caused by genetic bottlenecks or if they were replaced by later immigrants that never reached Norway.
Two star shaped patterns are apparent among the western European samples, separated by an A-G transition. One of these centres on NO1, which has been described before , while the other centres on the closely related NO4 and is made more apparent by our ancient samples. This could be an indication of two subgroups within the western European haplogroup, possibly reflecting different refugial areas in France and Iberia . Most of the European populations have undergone severe population reductions during recent centuries and several translocations which could have distorted any phylogeographic patterns within the haplogroup [6, 71]. A large-scale sampling of aDNA from other European populations would thus provide valuable insights into the phylogeography of European red deer.
Our results indicate that the current genetic diversity of Norwegian red deer can be explained by one post-glacial immigration event followed by a gradual loss of diversity and increasing population sub-division due to heavy exploitation and habitat fragmentation during the last two millennia. They also show that only females from the western European red deer lineage colonised Scandinavia, supporting previous findings that the eastern lineage had a more limited dispersal into Europe [14, 80]. The population decline seems to have been more prolonged in time than what is reflected in historic documents, indicating that even early human land use practices had an effect on red deer.
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We are very grateful to Hallvard Haanes and Anna Skog for providing additional info on the mtDNA diversity of the Norwegian red deer, and to Hallvard, John Linnell, Frank E. Zachos and an anonymous reviewer for valuable comments on the manuscript. We would also like to thank Liv Midthjell and Gro Bjørnstad for lab assistance, and Tore Fredriksen for help with providing samples. Funds were provided by the Directorate for Nature Management.
The authors declare that they have no competing interests.
JR did the laboratory work, analysed the data and drafted the manuscript. HKS performed the ABC-analyses. JR and AKH picked out the ancient samples. KHR supervised the laboratory work. All authors were involved in the study design, revising of the manuscript and read and approved the final manuscript.
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Rosvold, J., Røed, K.H., Hufthammer, A.K. et al. Reconstructing the history of a fragmented and heavily exploited red deer population using ancient and contemporary DNA. BMC Evol Biol 12, 191 (2012). https://doi.org/10.1186/1471-2148-12-191
- Ancient DNA
- Habitat fragmentation
- Mitochondrial DNA
- Genetic diversity
- Cervus elaphus