MHC class II DQB diversity in the Japanese black bear, Ursus thibetanus japonicus

Table 4 Gene diversities and nucleotide diversities for each conservation and management unit

		MHC DQBexon 2 region (267 bp)					mtDNA control region (Yasukochi et al. 2009)
	Conservation and management unit	No. of samples	No. of alleles	H _e	H _o	π _S	No. of samples	No. of haplotypes	h	π
Eastern Japan	Gassan-Asahi Iide (GAI)	21	4	0.48 ± 0.08	0.33	0.018 ± 0.009	6	5	0.93 ± 0.12	0.011 ± 0.008
	Echigo-Mikuni (EM)	13	7	0.79 ± 0.05	0.69	0.024 ± 0.011	16	7	0.82 ± 0.07	0.009 ± 0.006
	North-Central Alps (NCA)	12	10	0.85 ± 0.05 ^*	0.33 ^*	0.022 ± 0.010	23	8	0.85 ± 0.05	0.011 ± 0.007 ^d
	Hakusan-Okumino (HO)	36	18	0.76± 0.05 ^*	0.39 ^*	0.019 ± 0.008	74	8	0.72 ± 0.03	0.010 ± 0.006
Western Japan	Northern Kinki (NK)	23	8	0.68 ± 0.06	0.56	0.016 ± 0.008	96 (64)	4	0.75 ± 0.03	0.011 ± 0.007
	Eastern Chugoku (EC)	8	7	0.83±0.07^*	0.38^*	0.013 ± 0.007	31 (22)	2	0.25 ± 0.10	0.003 ± 0.002
	Western Chugoku (WC)	53	11	0.33 ± 0.06 ^*	0.19 ^*	0.005± 0.003	154 (33)	6	0.56 ± 0.04	0.005 ± 0.004
	Shikoku (SK)	9	6	0.73 ± 0.10^*	0.33^*	0.021 ± 0.010	11 ^c	2	0.55 ± 0.07	0.009 ± 0.006
	Overall	175	44	0.65 ± 0.03 ^*	0.36 ^*	0.016 ± 0.007	455	24	0.90 ± 0.01	0.013 ± 0.008

Bold character indicates conservation and management units which have more than 10 samples. Chokai Sanchi, Southern Ouu, Southern Alps and Kii Hanto units are excluded due to small number of samples. H_e: the expected heterozygosity; H_o: the observed heterozygosity. π_S or π: the nucleotide diversity. h: the haplotype diversity. ^*: a significant departure from Hardy-Weinberg equilibrium (P < 0.01). ( ): the number of haplotype data analyzed by [5]. ^a: the number includes subhaplotypes. ^b: the number was taken from the result of an identification of individuals using microsatellite analysis [90] because samples were obtained by hair trap. ^c: the number is samples that are newly added to samples used in our previous study [8]. ^d: the π value described in [8] (π = 0.001) was incorrect, and 0.01 was a correct value.

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