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Table 2 Primers and Taqman probes used in this study

From: Phenotypic plasticity in opsin expression in a butterfly compound eye complements sex role reversal

Gene Primers Taqman probe GenBank accession ID
UV opsin Forward primer: 5′-GCAAGCGAAGAAAATGAACGTAGAA-3′ 5′-CTGCCGCGTTTTGAT-3′ AF484248.1
Reverse Primer: 5′-CTATCCTGATTTCCGCTGACTCT-3′
blue opsin Forward primer: 5′-CGCGAGTGCAAGCATCTC-3′ 5′-TTGCCGTTCACCTTCC-3′ AY918894.1
Reverse primer: 5′-CACGAATTTTCCCCAGATCCTGAA-3′
LW opsin Forward primer: 5′-CGCCTGTGGAACCGATTACTT-3′ 5′-TTGCCACGACTTGTCG-3′ AY918895.2
  Reverse primer: 5′-AGCAGAAGATCGAGTAGAACAGGAT-3′   
  1. These sequences were used to quantitatively amplify B. anynana UV, blue, and LW opsin cDNAs. Primers and probes for eukaryotic 18S rRNA are proprietary (Applied Biosystems).