Results of Phylomapper analyses. Small circles indicate estimated location of ancestor of each clade from 100 replicate runs accounting for phylogenetic uncertainty in mtDNA gene tree. Squares indicate alternative ancestral locations used for hypothesis testing. Ancestral locations for B. nympha were not tested due to a lack of resolution in the mtDNA gene tree (see Methods). Red symbols: Bolitoglossa rufescens; blue symbols: B. occidentalis, pink symbols: B. nympha.