Quantification of teleost-specific Atlantic cod tlr s in response to temperature stress. Heatmap representing the expression of Atlantic cod tlrs in head-kidney and spleen in response to temperature stress. Adult fish were maintained at 4°C. After collecting initial control samples, the water temperature was gradually increased to 12°C in 4 h (4 hps) and the fish were maintained at this temperature for 72 h (72 hps). Relative expression of tlr21, tlr22 and tlr23 paralogues was quantified by qPCR as ratios between each sample and the initial control. Significance levels were set at P < 0.05 and statistically different expression values are enclosed in red boxes. Eef1a and ubi were used as internal controls.