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Table 2 Stresses performed on female-sterile strains S1-B12 and S3-A10 and the corresponding wild-type strains S1 and S3

From: Asexual reproduction induces a rapid and permanent loss of sexual reproduction capacity in the rice fungal pathogen Magnaporthe oryzae: results of in vitroexperimental evolution assays

  Proportion of female-fertile replicates before treatment Proportion of female-fertile replicates after treatments
Stress S1 S3 S1-B12 S1-A10 S1 S3 S1-B12 S3-A10
-80°C
(1 h)
5/5 5/5 0/5 0/5 5/5 5/5 0/5 0/5
-80°C
(24 h)
5/5 5/5 0/5 0/5 5/5 5/5 0/5 0/5
Mycelium
mashing
5/5 5/5 0/5 0/5 5/5 5/5 0/5 0/5
single-spore
isolation
1/1 1/1 0/1 0/1 12/12 12/12 0/12 0/10
growth on plant 1/1 1/1 0/1 0/1 7/10 10/10 0/8 0/10
  1. The treatments were: one hour at -80°C, 24 hours at -80°C, mycelium mashing by ultrasound, single-spore isolation and growth on plants. The ratios (number of replicates in which the strain was female-fertile)/(number of replicates in which the strain was female-sterile) are given before and after the various treatments