Asexual reproduction induces a rapid and permanent loss of sexual reproduction capacity in the rice fungal pathogen Magnaporthe oryzae: results of in vitroexperimental evolution assays

Table 4 Segregation of mating type and female fertility in the progenies of crosses between female-sterile evolved strains S1-B₁₂ and S3-A₁₀ and wild-type strains S3 and S1, and in backcross progenies

					Observed	segregation			Mat1:Mat2		f:s
Cross number	Parental	strains	Parental	phenotypes	Mat1.1-f	Mat1.2-f	Mat1.1-s	Mat1.2-s	Observed	Chi² and P (1:1)	Observed	Chi² and P (1:1)	Chi² and P (1:3)
	S1	S3	Mat1-f	Mat2-f	21	18	0	0	21:18	0.23 (0.631)	39:00
126	S1	S3-A10	Mat1.1-f	Mat1.2-s	6	3	15	10	21:13	1.88 (0.170)	12:24	7.53 (0.006)	0.04 (0.998)
130	S3	126/0/04	Mat1.2-f	Mat1.1-s	7	2	11	15	18:17	0.03 (0.866)	09:26	8.26 (0.004)	0.01 (0.922)
133	S1	126/0/35	Mat1.1-f	Mat1.2-s	9	4	8	15	17:19	0.11 (0.739)	13:23	2.78 (0.096)	2.27 (0.124)
127	S3	S1-B12	Mat1.2-f	Mat1.1-s	5	2	15	15	20:17	0.24 (0.622)	07:30	14.3 (0.000)	0.73 (0.866)
131	S1	127/0/25	Mat1.1-f	Mat1.2-s	13	18	6	6	19:24	0.58 (0.446)	31:12	8.40 (0.004)	50.9 (0.000)
132	S3	127/0/28	Mat1.2-f	Mat1.1-s	4	6	18	11	22:17	0.64 (0.423)	10:29	9.26 (0.002)	0.01 (0.926)

f: female-fertile, s: female-sterile (mutant). The control cross is the cross between the two wild-type strains S1 and S3 (first line). Values in bold indicate non significant differences at the 5% level

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