Figure 5
Resistance of parental, founding hybrid, and (A) temperature-selected or (B) ethanol-selected hybrid yeast to zymolyase. Cell wall dissolution was assayed by exposing yeast to zymolyase, placing them in spheroplast buffer at 37°C, then monitoring decrease in absorbance over time at λ = 600 nm, relative to T0. Each point is the mean (n = 3) of isolates from each selection vessel (1 from each vessel), 3 founding isolates, the F1, or parental isolates (Mean ± S.E.).