Table 1 Primers used for amplifying Mhc class I exon 3 in great tits
Primer | Sequence (5′ - > 3′) | PCR | Reference |
|---|---|---|---|
HN34 | CCATGGGTCTCTGTGGGTA | gDNA | [39] |
HN45 | CCATGGAATTCCCACAGGAA | gDNA | [39] |
T3F | TCCACACCATACAGCGAGTT | gDNA, cDNA | present study |
GBT3R | TTTACGCTCCAGCTCTTTCC | gDNA, cDNA | present study |
GTJSF | CGGCTGTGACCTCCTGTCC | gDNA | present study |
GTJSR | ATTCYGGGCAGATGTGCTT | gDNA | present study |
S2F | CACACCTCACAGTGGCTTTA | gDNA, cDNA | present study |
S2R | CAGCTCTTTCTGCCCATATC | gDNA, cDNA | present study |
L2F | CAATGGCTTTATGGCTGTGAC | gDNA, cDNA | present study |
L2R | CCAGCTCTTTCCTCCCGTAT | gDNA, cDNA | present study |
JSG2F | AGTTTCCGGCTGTGACCTC | gDNA | present study |
JSG2R | GCCCGTATTCGACGTATTTC | gDNA | present study |
JSG3F | CATACAGTGGCTTTATGGCTGT | gDNA | present study |
JSG3R | CCCGTATCCGGTGTATTTCC | gDNA | present study |
MHCV-F | CCCAGGTCTCCACACCATAC | gDNA | present study |
MHCV-R | AGCTCTTTCCGCCCGTATT | gDNA | present study |
MHCD-F | TTMYGGCTGTGACCTCCTG | gDNA, cDNA | present study |
MHCD-R | TTGCGCTYCAGCTCTTTC | gDNA, cDNA | present study |