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Table 3 Summary of interspecific comparisons for regional Viburnum samples

From: Barcoding success as a function of phylogenetic relatedness in Viburnum, a clade of woody angiosperms

Gene Region

Species

Aligned Length

Variable characters

Unique sequences

% Max ID rate

Max

Mean (SD)

> 1%

> 2%

Central America and Mexico (17 species)

       

matK

14

719

10

3

21.29

0.0126

0.0022 (0.0044)

14.29

0

rbcL

12

491

3

4

33.33

0.0041

0.0015 (0.0018)

0

0

rpl32-trnL

15

876

22

8

53.33

0.0208

0.0038 (0.0057)

13.33

0.95

trnH-psbA

16

410

17

10 (12)

62.50

0.0265

0.0084 (0.0065)

35.83

8.33

trnK

12

1057

24

7 (8)

58.33

0.0202

0.0047 (0.0066)

16.67

6.06

nrITS

17

602

77

14 (15)

82.35

0.0829

0.0213 (0.0222)

56.62

33.09

rbcL + matK

10

1210

5

4

40.00

0.0033

0.0010 (0.0012)

0

0

rbcL + matK + trnH-psbA

11

1612

15

7 (9)

63.63

0.0070

0.0025 (0.0018)

0

0

rbcL + matK + nrITS

11

1807

61

10

91.00

0.0232

0.0068 (0.0060)

20.00

3.63

Japan (16 species)

         

matK

13

725

24

10

76.92%

0.0155

0.0081 (0.0040)

32.05

0

rbcL

13

491

8

6

46.15%

0.0144

0.0065 (0.0048)

35.90

0

rpl32-trnL

13

873

46

11

84.62%

0.0266

0.0162 (0.0073)

76.92

35.90

trnH-psbA

13

439

41

10

76.92%

0.0477

0.0176 (0.0099)

85.90

34.62

trnK

14

1057

36

10

71.43%

0.0172

0.0093 (0.0043)

48.35

0

nrITS

13

608

104

13

100.00%

0.0914

0.0551 (0.0213)

97.44

89.74

rbcL + matK

13

1216

32

11

84.62%

0.0125

0.0074 (0.0032)

12.82

0

rbcL + matK + trnH-psbA

12

1654

65

10

83.33%

0.0157

0.0097 (0.0041)

71.21

0

rbcL + matK + nrITS

12

1823

134

12

100.00%

0.0372

0.0230 (0.0089)

86.36

69.70

  1. For the Viburnum species of Mexico and Central America and for Japan, the number of species analyzed, the aligned sequence length, the number of variable characters, the number of unique sequences with gaps treated as missing data (and with gaps treated as a fifth state), and the maximum number of species that can be identified by the data (Max ID rate = Identical sequences/total number of species) are reported. Summary statistics of genetic distances using a Kimura 2-parameter (K2P) model include: maximum genetic distance (max), mean interspecific distance (mean) with standard deviation (SD), and the proportion of comparisons of genetic distances greater than 1% (>1%) and greater than 2% (>2%).