Figure 5

VISTA plot of pairwise alignments for selected mammals in the region containing the PIGT , ELP/CTI and WFDC2 genes. Sequence homology within the PIGT-ELP/CTI-WFDC2 region of the dog, cow, elephant, horse, human, mouse and opossum genomes was determined with mVISTA [64]. The dog sequence was used as the reference sequence (horizontal axis, dog chromosome 24 numbering). Grey horizontal arrows indicate gene location and direction of transcription. Blue rectangles indicate coding exons and untranslated regions (UTRs) of the gene are depicted by light green rectangles. Exon 1 of canine WFDC2 was missing (gap in the current assembly) from the dog genome and is indicated by a blue rectangle with diagonal white stripes. The right axis indicates the percentage identity within a 100 bp window for each pairwise comparison, ranging from 10% to 100%. Regions sharing greater than 25% identity are shaded and the black horizontal line indicates 70% identity. The region containing the Kunitz domain-encoding ELP/CTI exon 2 was conserved in the cow, horse, mouse and opossum, but was absent in the elephant and human CTI genes (red boxes).