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Table 2 Average rates of synonymous (dS) and non-synonymous (dN) substitutions occurring in marsupial ELP and eutherian CTI

From: The mammary gland-specific marsupial ELP and eutherian CTI share a common ancestral gene

ELP/CTI protein-coding

region

dN

SE

dS

SE

dN/dS

Ratio

(a) Neutral selection test (dN ≠ dS)+*

(b) Purifying

selection test (dN < dS)+*

(c) Positive selection test (dN > dS)+*

Precursor protein

Marsupials

0.145

0.022

0.190

0.033

0.763

0.256 (NS‡)

0.117 (NS)

1.000 (NS)

 

Eutherians

0.194

0.026

0.225

0.033

0.862

0.232 (NS)

0.472 (NS)

1.000 (NS)

Mature protein

Marsupials

0.166

0.026

0.185

0.036

0.897

0.653 (NS)

0.334 (NS)

1.000 (NS)

 

Eutherians

0.186

0.028

0.242

0.039

0.786

0.273 (NS)

0.130 (NS)

1.000 (NS)

Signal peptide

Marsupials

0.071

0.029

0.226

0.094

0.314

0.133 (NS)

0.064 (NS)

1.000 (NS)

 

Eutherians

0.225

0.072

0.165

0.069

1.36

0.451 (NS)

1.000 (NS)

0.224 (NS)

N-terminus

Marsupials

0.240

0.064

0.116

0.048

2.07

0.064 (NS)

1.000 (NS)

0.041*

 

Eutherians

0.242

0.050

0.224

0.065

1.08

0.842 (NS)

1.000 (NS)

0.424 (NS)

BTPI KUNITZ 2 #

Marsupials

0.146

0.031

0.224

0.052

0.651

0.215 (NS)

0.101 (NS)

1.000 (NS)

 

Eutherians

0.162

0.035

0.243

0.054

0.667

0.200 (NS)

0.105 (NS)

1.000 (NS)

BPTI KUNITZ 1 ~

Marsupials

0.095

0.030

0.223

0.098

0.426

0.212 (NS)

0.103 (NS)

1.000 (NS)

 

Eutherians

0.066

0.026

0.264

0.110

0.250

0.122 (NS)

0.046*

1.000 (NS)

Trypsin interaction site ^

Marsupials

0.230

0.136

0.323

0.181

0.712

0.740 (NS)

0.363 (NS)

1.000 (NS)

 

Eutherians

0.175

0.093

0.228

0.131

0.768

0.689 (NS)

0.345 (NS)

1.000 (NS)

  1. #PS50279 153 nt, 51 aa.
  2. ~PS00280 57 nt, 19 aa.
  3. ^18 nt, 6 aa site from KU (NCBI cd00109).
  4. +Codon based Z-tests in MEGA5.
  5. *p < 0.05.
  6. ‡NS not significant.