Figure 4

The PARK7 SVA showed the ability to affect expression in a reporter gene construct. A – Schematic showing the genomic structure of the PARK7 SVA and the relationship to the fragments tested in the reporter gene constructs. B - The average fold activity of the different fragments from the SVA tested in both forward and reverse orientation over the minimal SV40 promoter alone (pGL3P) in the SK-N-AS cell line. Data was normalised to compensate for transfection efficiency, N = 4. C - The average fold activity in the MCF-7 cell line of the different fragments of the SVA in forward and reverse orientation over the minimal SV40 promoter alone (pGL3P) normalised to the internal control to account for transfection efficiency. N = 4. One tailed t-test was used to measure significance of fold activity of PARK7 SVA fragments over SV40 minimal promoter alone (pGL3P) and to compare fold activity of forward and reverse orientations. * P < 0.05, **P < 0.01, ***P < 0.001, # P < 0.05, ## P < 0.01, ### P < 0.001. N = 4.