Figure 4

Genetic variation based on 63 non-recombining ITS + 5.8S + D1/D2 haplotype sequences of Sebacina incrustans. Haplotypes are coded with H1 to H8 and heterozygous sequences are coded with a and b. (a) Maximum likelihood phylogenetic tree. Tree topology was computed from 1000 runs and was midpoint rooted. Bootstrap supports (>50%) are shown for each node. Substrate types where the basidiomata grew are mapped on the topology. iL1 to iL3 represent major lineages. (b) Median-joining network. Circle sizes are proportional to haplotype frequency and connecting lines are proportional to mutation events between haplotypes (numbers of mutated positions are given for all except one mutation. Colours indicate geographical areas where the basidiomata were collected. (c) Statistical parsimony network. Parsimony probabilities were set at 95%. Sizes of circular and rectangular areas are proportional to the number of individuals with that haplotype. Ectomycorrhizal tree families co-occurring in sampling sites are abbreviated as follows: B = Betulaceae, F = Fagaceae, P = Pinaceae.