High genetic diversity at the regional scale and possible speciation in Sebacina epigaea and S. incrustans

Table 1 Population statistics, diversity estimates and neutrality tests based on ITS + 5.8S + D1/D2 nucleotide sequences of Sebacina epigaea and S. incrustans

Species	n	h	s	hd	k	π	θω	Tajima’s	Fu & Li’s
Population								D	D*	F*
*S. epigaea*
BA	38	7	12	0.783	3.691	0.024	0.018	1.04	1.48*	1.57
WA	6	4	4	0.679	2.214	0.014	0.010	1.90	1.31	1.59
EA	2	1	0	0.000
NV	3	2	13	0.500	6.500	0.042	0.046	−0.84	−0.84	−0.86
HO	1	1	1	1.000	1.000	0.006	0.006
All	50	11	53	0.853	4.962	0.032	0.044	−0.87	1.07	0.42
*S. incrustans*
BA	22	13	23	0.059	9.124	0.020	0.013	2.02	1.67*	2.09*
WA	6	3	7	0.667	3.238	0.007	0.001	0.69	0.60	0.67
EA	7	5	9	0.769	3.615	0.008	0.001	0.97	0.96	1.09
NV	5	3	18	0.700	7.600	0.016	0.019	−0.89	−0.89	−0.95
HO	8	2	25	0.429	10.714	0.023	0.021	0.59	1.61*	1.52
All	48	8	46	0.755	11.341	0.024	0.021	0.54	1.82*	1.60

Sampling areas are coded as follows: BA = Bavarian Alps, WA = Western Swabian Alb, EA = Eastern Swabian Alb, NV = Neckar Valley, HO = Hohenlohe. n = sample size, h = number of haplotypes, s = number of segregating sites, hd = haplotype diversity, k = average number of nucleotide pairwise differences, π = number of nucleotide differences per site, θω = Watterson’s estimate of ϕ per site. Significant tests (P < 0.05) are marked with *.

ISSN: 2730-7182