Figure 4
Fluorescent in situ hybridization of C. baculoides symbiont cells. (A) Abdominal image of an adult male. Signals of the symbiont cells are detected in bacteriocyte clusters located on both sides of the abdominal body cavity. White squares indicate the areas of panels B and C. (B, C) Enlarged images of the bacteriocyte clusters in panel A. Signals of the symbiont cells are localized in the cytoplasm of the bacteriocytes. (D) Abdominal image of an adult female. Signals of the symbiont cells are localized in ovarial ampullae located at the base of the ovaries. (E) An enlarged image of an ovarial ampulla. (F) A snapshot of symbiont transmission from an ovarial ampulla to a developing oocyte. Red and green signals indicate symbiont 16S rRNA and host nuclear DNA, respectively. Abbreviations: oa: ovarial ampulla, ov: ovariol, vg: vagina, pp: posterior pole of oocyte.