Evolution at increased error rate leads to the coexistence of multiple adaptive pathways in an RNA virus

Table 2 Substitutions that remain as polymorphisms at transfer number 90 in the bacteriophage Qβ evolved in the presence of AZC

Substitution	Gene¹	change²	First detected³
U1520C	Coat	Syn	50
C1604G	Coat	Syn	60
C2059G	Read-through	T/S	60
A2277G	Read-through	K/E	90
C2378G	Replicase	N/K	90
C2384(G+U)	Replicase	Syn	60
C2471G	Replicase	Syn	30
A2982G	Replicase	T/A	50
C3413(U+G)	Replicase	Syn	50
C3545A	Replicase	Syn	70
U3582C	Replicase	Y/H	50
C3879G	Replicase	L/V	70
G3945A	Replicase	G/S	40
U3989C	Replicase	Syn	30
A4006G	Replicase	K/R	60

¹Location of each substitution in the genome of the bacteriophage Qβ.
²Type of change produced by each nucleotide substitution. In the case of non synonymous changes, the amino acid substitution is indicated.
³Transfer number at which each substitution was first detected as a double band in the chromatogram corresponding to the consensus sequence of that population. The genomic region analyzed comprised from nucleotide 180 to 4180.

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