Skip to main content

Table 4 Relative fitness of bacteriophage Qβ site-directed mutants

From: Evolution at increased error rate leads to the coexistence of multiple adaptive pathways in an RNA virus

Site-directed mutant Relative fitness1 Relative fitness1 Dominant virus in competition with Qβwt2
  +AZC -AZC +AZC -AZC
A1746U 0.71 ± 0.02* 0.58 ± 0.03* A1746U wt
A2982G 1.24 ± 0.08* 0.89 ± 0.05* A2982G wt
U3582C 1.31 ± 0.03* 0.89 ± 0.06* U3582C wt
C3413G3 0.95 ± 0.04* 0.92 ± 0.07* C3413G +wt (transfer 15) C3413G +wt (transfer15)
G3945A3 1.1 ± 0.06* 1.02 ± 0.05 G3945A (transfer15) G3945A (transfer15)
U3989C 0.79 ± 0.04* 0.88 ± 0.05* wt wt
  1. 1Relative fitness was evaluated with respect to the virus Qβwt either in the absence or the presence of AZC (60 μg/ml). The absolute fitness value estimated for Qβwt was 15.42 ± 0.50 in the absence of AZC and 9.68 ± 0.22 in its presence. The asterisk indicates that the difference between the fitness values of the site-directed mutant and the virus Qβwt was significant (p value < 0.05, Student’s t test for the difference of means).
  2. 2Competition between the site-directed mutants indicated and the virus Qβwt was carried out as described in Methods during 5 transfers either in the absence or the presence of AZC (60 μg/ml).
  3. 3Since no virus became dominant at transfer number 5, competitions carried out with viruses QβC3413G and QβG3945A were extended until transfer number 15, yielding the results shown in the table.