Figure 4

Double fluorescent in situ hybridization of PfXlox with PfCdx or PfGsx . DNP-labeled PfXlox probe and DIG-labeled PfCdx probe were used for in situ hybridization on mid-gastrula (A-C), late gastrula (D-F), and tornaria larva (G-I). The arrows and arrowheads indicate the anterior boundaries of the PfCdx and PfXlox expression domain, respectively. Double stainings of PfGsx and PfXlox transcripts were performed on the late gastrula embryos (J-L).