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Table 3 DAVID gene ontology functional annotation enrichment analysis in magnetic simulators

From: Suboptimal evolutionary novel environments promote singular altered gravity responses of transcriptome during Drosophilametamorphosis

GBF

ΔT

↓O2

G level

Affected genes (limma raw(FDR))

Behaviour responses

Metamorphosis/morphogenesis/organogenesis

Stress responses

GO clusters observed also in control conditions

 

Reproductive/ mating/oviposition

Sensorial/ hormonal/ odorant prot.

 

Abiotic (heat, light & hypoxia)

Biotic (defense & immune sys.)

Insect cuticle proteins

Cytoesqueleton and cell adhesion

Proteolysis

Red-Ox states/energy

Hemo/ metals/ions binding

Mag

No

Yes

0g* UP

61(0)

  

4.5×10-2

   

3.8×10-2

   
   

0g* Down

62(0)

1.7×10-19

      

6.2×10 -4

  
   

1g* UP

17(0)

          
   

1g* Down

25(0)

       

3.4×10-2

  
   

2g* UP

420(30)

6.4×10-2

8.8×10-3

2.1×10 -4

1.3×10-2

1×10-2

5×10-8

6.7×10 -4

  

3.1×10-3

   

2g* Down

328(42)

6.1×10-22

1.2×10 -5

  

6.6×10-2

  

4.4×10 -6

2.9×10-8

 
 

Yes

Yes

0g* UP

635(458)

1.9×10 -4

7.7×10 -5

 

1.9×10-3

2.7×10-2

  

1.1×10 -5

4.2×10-12

1.7×10 -6

   

0g* Down

524(349)

2×10-2

2.3×10-3

 

3.4×10-2

1.5×10-2

2×10-25

 

2.9×10-3

7.2×10-11

4.7×10 -5

   

1g* UP

350(196)

 

2.9×10-3

 

1×10-3

1.4×10-3

  

1.1×10-2

1.1×10-11

2.9×10-8

   

1g* Down

191(97)

     

2.5×10-18

 

2.7×10-2

  
   

2g* UP

208(7)

    

1.4×10-2

   

1.2×10-6

8.6×10 -5

   

2g* Down

157(2)

     

2.3×10-15

 

6.8×10-2

  
  1. The number of up- or down-regulated probesets in each GBF facility (first column) and condition (column 2 to 4) has been calculated and is shown in the fifth column (filtering with a p-value limma < 0.001). Relative enrichment analysis of these gene lists have been done, and the more frequent GO groups observed in experimental (columns 6 to 10), or 1g/environmental controls (columns 11 to 15) are included. Statistically meaningful p values are indicated in the table using italics and bold fonts to emphasize a greater level of enrichment in each particular GO group is observed. * indicates the presence of a high magnetic field.