Figure 2

Karyotypes and localization of 35S (green) and 5S (red) rDNA loci in diploids of Prospero . (A) P. obtusifolium; (B) P. hanburyi; (C–I) P. autumnale complex: (C) AA; (D) B⁷B⁷; (E) B⁷B⁷ with duplicated 5S rDNA locus in chromosome 1; (F) B⁵B⁵; (G) B⁶B⁶; (H) B⁶B⁶ with homozygous NOR translocation (NOR in pair of chromosome 6); (I) B⁶B⁶ with heterozygous NOR translocation (NOR in one of each chromosome 3 and 6). Insets in (A) and (G) show chromosomes of a single cell which were lying at some distance from the main chromosome group and either could not be photographed together using high magnification objectives or were too far apart to clearly demonstrate chromosome morphology while showing the whole field. Scale bar = 5 μm.