Chromosomal diversification and karyotype evolution of diploids in the cytologically diverse genus Prospero(Hyacinthaceae)

Table 2 Genome size, karyotype length and rDNA loci number and localization in Prospero

Cytotype	5S and 35S rDNA loci number and localization^{1, 2}	Genome size	Chromosome size
(Accession number)	5S and 35S rDNA loci number and localization^{1, 2}	1C (pg) ± SD	HKL (μm) ± SD
Prospero hanburyi
(H397)	5S (L-P^chr1)	6.81 ± 0.017	44.90 ± 4.04
(H397)	35S (S-ST^chr2)	6.81 ± 0.017	44.90 ± 4.04
P. obtusifolium
(H540)	5S (L-P^chr2, L-P^chr3, S-P^chr3)	4.94 ± 0.039	29.01 ± 0.77
(H540)	35S (S-P^chr3)	4.94 ± 0.039	29.01 ± 0.77
P. autumnale complex
AA
(H551)	5S (S-P^chr2)	7.85 ± 0.045	48.35 ± 7.15
(H551)	35S (L-P^chr3)	7.85 ± 0.045	48.35 ± 7.15
B⁷B⁷
(H450)	5S (L-D^chr1)	4.23 ± 0.048	33.76 ± 1.45
(H450)	35S (L-P^chr3)	4.23 ± 0.048	33.76 ± 1.45
(H424)	5S (L-D^chr1)	4.45 ± 0.023	28.70 ± 1.74
(H424)	35S (L-P^chr3)	4.45 ± 0.023	28.70 ± 1.74
B⁶B⁶
(H274)	5S (L-D^chr1, S-P^chr2)	6.27 ± 0.083	38.34 ± 1.24
(H274)	35S (L-P^chr3)	6.27 ± 0.083	38.34 ± 1.24
B⁵B⁵
(H640)	5S (L-D^chr1)	4.86 ± 0.002	29.67 ± 2.58
(H640)	35S (S-P^chr3)	4.86 ± 0.002	29.67 ± 2.58
Translocations
B⁶B⁶	5S (L-D^chr1, S-P^chr2)	6.05 ± 0.011	34.97 ± 3.98
(H197)	35S (S-P^chr6)	6.05 ± 0.011	34.97 ± 3.98
B⁶B⁶	5S (L-D^chr1, S-P^chr2)	6.07 ± 0.031	30.03 ± 1.99
(H340)³	35S (L-P^chr3, S-P^chr6)	6.07 ± 0.031	30.03 ± 1.99

¹ L, long arm; S, short arm; D, distal (interstitial) location of 5S rDNA; P, pericentric location of 5S or 35S rDNA; ST, subterminal location of 35S rDNA; ^chr, number of the chromosome.
²Scale bar = 1 μm; only the chromosomes bearing rDNA are shown: filled and open circles indicate position of 35S and 5S rDNA loci, respectively.
³Heterozygote; both chromosomes carrying 35S rDNA (chromosome 3 and chromosome 6) are shown.

ISSN: 2730-7182