Figure 3From: A novel type of light-harvesting antenna protein of red algal origin in algae with secondary plastidsLocalisation of the RedCAP protein in complex plastids of diatoms. (A) Bipartite plastid targeting sequences in photosynthetic Chromista. The conserved “ASAFAP”-motif [32, 33] at the interface between signal and transit peptides is marked. (B) Sequence of the Phaeodactylum tricornutum RedCAP full length GFP fusion construct. (C) Expression of the full-length RedCAP:GFP fusion constructs in P. tricornutum. (D) Expression of GFP without targeting pre-sequence in P. tricornutum. Panels show microscopical images of transmitted light (differential interference contrast, DIC), chlorophyll autofluorescence, GFP fluorescence and a merged image from left to right, fluorescence images are maximum intensity projections of 14 slices of a 4.9 μm image stack, scale bars represent 10 μm.Back to article page