- Research article
- Open Access
Where does Neisseria acquire foreign DNA from: an examination of the source of genomic and pathogenic islands and the evolution of the Neisseria genus
© Putonti et al.; licensee BioMed Central Ltd. 2013
- Received: 29 April 2013
- Accepted: 28 August 2013
- Published: 4 September 2013
Pathogenicity islands (PAIs) or genomic islands (GEIs) are considered to be the result of a recent horizontal transfer. Detecting PAIs/GEIs as well as their putative source can provide insight into the organism’s pathogenicity within its host. Previously we introduced a tool called S-plot which provides a visual representation of the variation in compositional properties across and between genomic sequences. Utilizing S-plot and new functionality developed here, we examined 18 publicly available Neisseria genomes, including strains of both pathogenic and non-pathogenic species, in order to identify regions of unusual compositional properties (RUCPs) using both a sliding window as well as a gene-by-gene approach.
Numerous GEIs and PAIs were identified including virulence genes previously found within the pathogenic Neisseria species. While some genes were conserved amongst all species, only pathogenic species, or an individual species, a number of genes were detected that are unique to an individual strain. While the majority of such genes have an origin unknown, a number of putative sources including pathogenic and capsule-containing bacteria were determined, indicative of gene exchange between Neisseria spp. and other bacteria within their microhabitat. Furthermore, we uncovered evidence that both N. meningitidis and N. gonorrhoeae have separately acquired DNA from their human host. Data suggests that all three Neisseria species have received horizontally transferred elements post-speciation.
Using this approach, we were able to not only find previously identified regions of virulence but also new regions which may be contributing to the virulence of the species. This comparative analysis provides a means for tracing the evolutionary history of the acquisition of foreign DNA within this genus. Looking specifically at the RUCPs present within the 18 genomes considered, a stronger similarity between N. meningitidis and N. lactamica is observed, suggesting that N. meningitidis arose before N. gonorrhoeae.
- Horizontal gene transfer
- Pathogenicity islands
- Pathogen-host DNA transfer
Infectious diseases, which remain a major cause of human morbidity/mortality, are the direct result of interactions between the human host and unique sets of microbial virulence factors. These virulence factors have often been found in clusters of horizontally transferred 10–100 kb genomic DNA regions [1–3] or in short scattered virulence-associated 1–10 kb islets . Regions of DNA introduced through horizontal transfer, referred to as genomic islands (GEIs), can encode for genes of a variety of functions. Identification of the subset of GEIs that encode for virulence factors, referred to as pathogenicity islands (PAIs), can lead to the understanding of the organism’s pathogenicity within the host.
There is considerable interest in developing statistical methods to predict PAIs and/or GEIs for future experimental validation as well as furthering our understanding of pathogenicity. As such, numerous statistical methods have been developed to identify aberrant regions in terms of nucleotide composition [5–20] as well as examining phylogenetic discrepancies [21–27]. While alone each of these techniques is appropriate for the detection of a particular GEI/PAI feature, no single approach is capable of detecting all GEIs and PAIs. Combinatorial approaches, e.g. [28–33], although often more cumbersome and inconclusive (in the case where a region is identified by just some of the measures used), can discover more GEIs and PAIs. Beyond just detecting GEIs and PAIs is the task of identifying the source of the genes acquired. This is further complicated when looking at species with open pan-genomes (in which new strains always include novel genes) such as Neisseria meningitidis[34–36].
A graphic approach called Similarity Plot or S-plot for rapid large scale comparison, analysis, and visualization of genomic sequences was previously employed to recognize regions within a genomic sequence that originated through horizontal gene transfer as well as genes under specific selective forces . This tool, as described in detail in our previous work , identifies regions of unusual compositional properties (RUCPs) via a sliding-window approach. Recently we expanded this functionality, permitting gene-by-gene comparative analyses to be performed.
Herein we present the results of a comparative analysis of all complete annotated Neisseria genomes currently available. This includes 18 genomes: three from the species N. gonorrhoeae, 14 from the species N. meningitidis and one from the non-pathogenic species N. lactamica. As previous analysis has shown, N. meningitidis exhibits a moderately strong codon bias effect [38, 39]. As such, recently acquired genes will likely exhibit a codon usage more attuned to the tRNA abundances within their native genome. Through the identification of horizontally acquired elements, we can better understand the evolution of this genus with respect to the transformations occurring at the genomic level as well as the variations in virulence between the species and strains. Looking specifically at the RUCPs present within all 18 genomes considered, a stronger similarity between N. meningitidis and N. lactamica is observed, suggesting that N. meningitidis arose before N. gonorrhoeae as shown in previous studies .
Visualizing similarities and dissimilarities in compositional properties within Neisseriaspp
Number of RUCPs identified for each Neisseria strain
N. gonorrhoeae FA 1090
N. gonorrhoeae NCCP11945
N. gonorrhoeae TCDC-NG08107
N. meningitidis Z2491
N. meningitidis WUE 2594
N. meningitidis MC58
N. meningitidis alpha710
N. meningitidis G2136
N. meningitidis M01-240149
N. meningitidis M04-240196
N. meningitidis H44/76
N. meningitidis M01-240355
N. meningitidis NZ-05/33
N. meningitidis FAM18
N. meningitidis 053442
N. meningitidis 8013
N. meningitidis alpha14
N. lactamica ST-640
Examination of the 18 Neisseria genomes was conducted using both a window-by-window approach, with a window size of 5000 nucleotides, as well as a gene-by-gene approach. (See Methods for a detailed description of each). We refer to the regions exhibiting unusual compositional properties from the window-by-window analysis as wRUCPs and from the gene-by-gene analysis as gRUCPs. Windows/genes can exhibit unusual compositional properties as a result of horizontal gene transfer or specific selective forces. The number of wRUCPs and gRUCPs varied from strain to strain as well as serogroup to serogroup. In total 414 wRUCPs and 2,172 gRUCPs were identified (Table 1). While the N. meningitidis WU 2594 strain (serogroup A) isolated from a patient suffering from acute bacterial meningitidis  contained the fewest wRUCPs, 144 genes were identified as having unusual compositional properties; analysis of the location of these genes revealed that there were several clusters of adjacent genes. While some of the gRUCPs include genes which have been assigned a particular gene name and/or function, the majority (77%) are annotated as hypothetical proteins. The complete listing of the wRUCPs and gRUCPs can be found in Additional file 6: Table S1 and Additional file 7: Table S2, respectively. Within the 414 windows identified, 715 (32%) of the genes within these windows were also identified by the gene-by-gene analysis, indicative of larger acquisitions of gene clusters within the acquired DNA fragments. Thus, the gene-by-gene analysis provides a finer granularity for detecting regions of interest.
Identifying gRUCPs shared within the Neisseriaspp
Summary of homologous genes conserved amongst and within only the pathogenic Neisseria strains, the N. meningitidis strains, and the N. gonorrhoeae strains
# Homologous genes
Genes found in other genera
Pathogenic Neisseria strains
• Putative phage associated protein present in a few Burkholderia spp.
• Annotated in other species, e.g. Moraxella catarrhalis and Haemophilus influenzae, as ribonuclease T (rnt)
N. meningitidis strains
• Putative heavy metal transport protein (NMAA_1445)
• PEMK-like protein
• Superoxide dismutase*
• Putative heavy metal transport protein*
• Hypothetical protein*
• FrpC protein*
N. gonorrhoeae strains
• 4 phage-associate proteins
• Hypothetical proteins *
Numerous gRUCPs were present in many but not all of the genomes for a species, indicating strain-specific losses. This was most clearly seen within the N. mening-itidis sequences studied. The N. meningitidis strains contained 88 gRUCPs that were present within the N. lactamica genome and absent from the three N. gonorrhoeae genomes. In contrast, there were only four gRUCPs present within the N. gonorrhoeae genomes and the N. lactamica genome that were not present in any of the N. meningitidis strains. Under the premise that all three of these species derived from the same ancestor [46, 47], the presence/absence of N. mening-itidis and N. gonorrhoeae gRUCPs in N. lactamica presents insight into their evolutionary history. Either N. gonorrhoeae lost the gRUCPs present within the N. meningitidis strains or N. meningitidis acquired these gRUCPs from N. lactamica post-speciation. As previous research has found that there is some genetic exchange between N. lactamica and N. meningitidis[48, 49], the former – the loss of gRUCPs by N. gonorrhoeae – is more plausible than the latter.
PAI presence in RUCPs
Some of the genes which have been previously identified as Neisseria virulence factors (VFDB) that were found as RUCPs in the N. meningitidis strains examined
Major virulence factors:
Sampling of genes found as RUCPs in N. meningitidis strains:
Adherence (LOS, Type IV pili)
NMBB_0072, NMBB_0073, NMBB_0073A
Invasion (Opa, Opc, Porin)
NMBB_1866, NMC1877, NMBNZ0533_1971
Iron uptake (FbpABC, HmbR, HpuAB, Lbp, Tbp)
Our analysis additionally recognized genes which have been associated with functionality that enables virulence. gRUCPs include the maf family of proteins which play a role in adhesion  as well as many of the comE gene copies which are involved in DNA uptake [56, 57]. The RUCPs also contain numerous other proteins involved in the type IV secretion system, including the tra family of proteins and ltgX, amongst others. T4SS genes are often identified by their homology to the transfer genes of conjugative plasmids or the Ti plasmid of Agrobac-terium tumefaciens. The gene atlA is classified as a RUCP; this gene has been found to play a critical role in bacterial resistance to phagocytosis and survival in the bloodstream in Streptococcus mutans. Providing similar protection from phagocytosis , several of the RUCPs are or include genes annotated as Cu-Zn superoxide dismutases (Additional file 6: Table S1 and Additional file 7: Table S2).
Ascertaining the acquisition and putative sources for gRUCPs
Of the gRUCPs found, 217 appear to be unique to a single Neisseria strain (R2 > 0.9): 80 in N. gonorrhoeae strains, 99 in N. meningitidis strains, and 38 in the N. lactamica genome. The nucleotide sequence of each was then BLASTed against the nr/nt Nucleotide database in an effort to find the putative source of these genes. Of the 217 genes, only 84 (17 N. gonorrhoeae, 60 N. meningitidis and 7 N. lactamica) produced significant similarities (Additional file 13: Table S7). (As a result of these BLAST searches, four were found to be annotated in other genera as 50S ribosomal protein L36, although not in Neisseria, and thus removed from further analysis.) In total, 80 of these 213 genes identified as exhibiting unusual compositional properties and unique to a single Neisseria strain are present in the genomes of other organisms. Thus, these genes could either be acquired via horizontal gene transfer or their unusual compositional properties are the result of locus specific selective forces, also at play in other bacterial genomes. The remaining 64% of these genes do not show significant sequence similarity to any sequenced species in NCBI’s nucleotide collection.
The BLAST hits were comprised of several hypothetical proteins as well as putative phage associated proteins from a wide variety of bacteria. Homologs to other bacterial species were also identified with annotations including: Cu-Zn superoxide dismutase, excinuclease, chaperone protein DnaK, SecY subunit, zinc transporter, and cation efflux protein. Several of these hits were to other human bacterial pathogens, e.g. Escherichia coli, Helicobacter cinaedi, Salmonella enterica, Haemophilus influenzae, Bordetella pertussis, Pseudomonas aeruginosa and Rickettsia, amongst others (Additional file 13: Table S7). Moreover, many of the BLASTn hits of the N. meningitidis gRUCPs were to bacteria containing a capsule, including Pseudomonas spp., Kiebsiella spp., Haemophilus spp., Escherichia spp., Salmonella spp. and Bordetella spp.
Top 10 genes exhibiting a composition most divergent from their genome’s respective norm
Integral membrane protein
FrpA/C-related protein, truncated
From our examination of the gRUCPs found to be unique to individual Neisseria strains, a number of putative sources, including other pathogenic bacteria, were identified suggesting that gene exchange occurs between Neisseria spp. and other bacterium within their microhabitat. Furthermore, gRUCPs identified within N. meningitidis strains were homologous to a number of other bacterium containing a capsule. The homology identified between a N. meningitidis gRUCP and the human genome revealed for the first time that N. meningitidis may be capable of integrating host DNA. The majority of the gRUCPs found to be unique to individual strains, however, did not BLAST to any known species or gene. While the evolutionary path of the Neisseria genus has long been a point of debate, our examination of the gRUCPs, which includes the pathogenome of Neisseria (Table 3), suggests that N. meningitidis is more closely related to the non-pathogenic Neisseria species than N. gonorrhoeae.
This study is consistent with previous analyses of the N. meningitidis genomes finding the species to have an open pan-genome . The approach employed here permits one to easily identify those genes most divergent to the underlying composition of the genome. Genes which are unique and likely recent acquisitions for even the non-pathogenic N. lactamica genome were observed signifying that the three Neisseria species are all recipients of horizontally transferred elements post-speciation. Furthermore, evidence of strain specific and serotype specific acquisitions of genes were also identified. For instance, the N. meningitidis MC58 and N. gonorrhoeae NCCP11945 genomes have a number of unique gRUCPs, suggesting that these two strains have acquired genes unique to their individual evolutionary history.
The majority of the genes which are unique to a particular strain did not reveal statistically significant homologies with any sequenced species indicating that the source of these proteins remains unknown. Because these gRUCPs exhibited divergent nucleotide compositions, it is not likely that the genes are native to Neisseria. Rather, we hypothesize that they were acquired from another genus. For those gRUCPs which were found to be strain specific and homologous to genes within other sequenced bacterial genomes, one of two scenarios is possible: (1) the species identified from the BLAST search can be the source of the gene acquired by Neisseria or (2) both species could have acquired the gene from the same source. Looking at some of the organisms identified from the BLAST searches (Additional file 13: Table S7), one can find very probable instances of gene exchange. For instance, many of the BLAST results find similarities with sequences in Haemophilus spp. and Moraxella catarrhalis, other human pathogens.
The sequence similarity between gRUCPs and eukaryotic species was unexpected. While one of these gRUCPs has recently been discussed in the literature , the acquisition of human DNA was previously thought exclusive to N. gonorrhoeae. The BLAST result finding homology between the N. meningitidis gene NMB1848 and a portion of the PHD finger protein sequence PHF21B is the first report to our knowledge indicating human DNA uptake within this species. The region (195 nucleotides in length) is an annotated conserved domain, Formin Homology Region 1 characteristic of its low complexity repeats of around 12 residues . This domain is not found within the genome sequences of either N. gonorrhoeae or N. lactamica. In fact, save a moderate homology with a sequence within the B. thuringiensis genome (Figure 3B), this domain appears to be eukaryotic in origin. The complete NMB1848 coding region of the MC58 strain is also present in other N. meningitidis strains, although not annotated as a coding region; therefore it was not identified as a gRUCP in the other N. meningitidis strains. Thus, we hypothesize that it was acquired post-speciation with N. gonorrhoeae and from a eukaryotic source, most likely its host. As such, the acquisition of host DNA is not exclusive to N. gonorrhoeae.
The recent sequencing of the non-pathogenic N. lactamica genome facilitates the identification of genes responsible for the pathogenicity of N. gonorrhoeae and N. meningitidis. Our analysis revealed two hypothetical proteins which are unique to the pathogenic Neisseria; further investigation is needed to ascertain if or how they are contributing to virulence. The gRUCPs conserved amongst all N. meningitidis strains and absent from N. gonorrhoeae and N. lactamica include several genes of interest, many of which are annotated as superoxide dismutase. Previous studies have found that N. mening-itidis uses these enzymes to neutralize the effect of reactive oxygen species within the host [64, 65]. Furthermore, in a recent transcriptomic study, these genes were found to be upregulated during infection . In congruence with previous literature , our analysis confirms the importance of metal transport proteins for N. meningitidis as they are conserved amongst all of the N. meningitidis strains examined. The presence of the PEMK-like protein within the N. meningitidis genomes and absent from the N. gonorrhoeae genomes suggests that this may also aid in the meningococcal infection, by interfering with host mRNA .
The composition-based method employed here was able to identify virulence genes previously identified within the pathogenic Neisseria spp. [3, 7, 28, 49–54]. Included are the capsule genes, some of which are present in N. gonorrhoeae as well as non-pathogenic species of Neisseria (Additional file 5: Figure S5). The presence of these cps-associated genes in N. gonorrhoeae, non-pathogenic Neisseria spp., as well as non-invasive meningococcal strains has led others to suggest that these genes have a biological role distinct from pathogenicity. N. lactamica’s capsule is antigenically similar to that of N. meningitidis. The pilin gene was identified as a gRUCP for the non-pathogenic N. lactamica. Despite its important role in pathogenicity, the presence and structure of the pilin does not indicate the species’ ability to cause human disease . The identification of the pilin genes as gRUCPs in the non-pathogenic as well as pathogenic Neisseriae suggests that selection is playing a role in shaping the composition and function of this gene cluster .
Because the vast majority of the RUCPs in N. lactamica are also present within the genomes of the pathogenic species, one may conclude that the two pathogenic species are more closely related to each other than either is with the non-pathogenic N. lactamica. This mirrors previous phylogenic analysis of the Neisseria spp. using a small set of homologous genes [46, 47]. Unraveling the evolutionary history of the emergence of N. gonorrhoeae and N. meningitidis is far from trivial. Several genes were identified as acquisitions exclusive to N. gonorrhoeae or N. meningitidis. Moreover, genes that are present in one, two or three of the N. gonorrhoeae genomes and are present within all (or the majority) of the N. meningitidis strains examined here suggest their acquisition prior to the divergence of the two species. More baffling are the instances in which a gene is present in a single N. gonorrhoeae genome and in just a few (and different serotypes) of the N. meningitidis strains. Looking specifically at the gRUCPs present within all 18 genomes considered, a stronger similarity between N. meningitidis and N. lactamica is observed. If these gRUCPs were acquired prior to speciation of N. lactamica and the pathogenic species, this would suggest that N. meningitidis is more closely related to the non-pathogenic N. lactamica[40, 48, 49]. The availability of signifi-cantly more N. meningitidis genomes, however, is likely contributing to this observation. Further sequencing and annotation of N. gonorrhoeae genomes as well as additional Neisseria spp. is underway and will likely shed further light on the evolution of the genus.
The approach employed here presents a new means by which investigators can readily identify genes unique to a species or strain as well as identify genes which exhibit compositional properties aberrant from the genome norm such as acquired elements and genes under unique selective pressures. Analysis of the identified GEIs revealed that gene exchange occurs frequently between Neisseria spp. and other bacteria within their microhabitat; it also occurs with their human host. From the genic material acquired by the 18 strains examined here, albeit not a comprehensive representation of the strains present in nature, it appears that the N. meningitidis strains are more closely related to the non-pathogenic Neisseria lactamica than the N. gonorrhoeae strains are.
The genes identified as exclusive to the pathogenic Neisseria genomes, N. meningitidis genomes, or N. gonor-rhoeae genomes provide insight into the genus both from an evolutionary perspective as well as from the perspective of that of a microbiologist. Given the prevalence of the pathogenic species, identifying additional factors which aid in the virulence of Neisseria is of importance. Genes involved in iron and zinc uptake and downstream processes have been thoroughly investigated as candidates for the development of vaccines (e.g. [71, 72]). Included in the RUCPs identified here are a number of hypothetical proteins which warrant further investigation as putative virulence factors.
Neisseria genome sequences
The complete genomes of three N. gonorrhoeae, 14 N. meningitidis, and one N. lactamica strains were obtained from NCBI’s FTP site. All of these genomes have been assembled and annotated [34, 36, 43, 54, 55, 73–79]. The annotation files for all these genomes were collected (*.ptt and *.rnt files). Table 1 lists the genomes.
Quantifying genomic similarity and dissimilarity
The compositional profile of each window, in the case of the sliding window approach, or each gene, in the case of the gene-by-gene approach, was computed as previously described  and summarized as follows. Looking at an individual window or gene sequence, the frequency of occurrence of all k-mers is determined. When evaluating the similarity/dissimilarity of two windows or two genes, these two frequency profiles can be compared. While a variety of different methods can be implemented, here we quantify distance according to the coefficient of determination (R2). The distribution P(S) of appearances of all possible k-mers inside a given window is P(S) = N S /(w − k + 1), where N S and w are, correspondingly, the number of appearances of k-mer S and the total number of k-mers in the window being examined. The correlation statistic is appropriate only when representative statistics are collected such that N s > 1; therefore one must impose the condition w > 4 k when taking either the sliding window or gene-by-gene approach.
Analyses were conducted for a variety of different sizes of k meeting the condition w > 4 k . For the window-by-window analysis with a window size of 5000 nucleotides, k = 6 was used. wRUCPs identified for this k-mer size were also found when k = 5 and k = 4. Because the length of genes varies across the genome, the gene-by-gene analysis necessitated a choice of k such that 4 k is smaller than the shortest annotated coding region. As such, the largest size k which can be used is 3. Because our analysis focuses on 3-mer and 6-mer compositional profiles (capturing the underlying codon usages), genes which do not exhibit the Neisseria codon usage and are likely from foreign sources are identified as exhibiting an unusual composition.
To assess the similarity of the compositional properties of a particular window i within its genomic sequence, the average R2 value as well as the standard deviation is calculated. Thus, windows which exhibit the same k-mer usage profiles will have a R2 value approaching 1. Windows containing regions which have not been under strong selection to follow the genome-wide composition (e.g. mono- or dinucleotide biases or codon biases), such as rRNA, will be less similar to the other windows in its genome, thus resulting in a smaller R2 value. Likewise, foreign DNA recently integrated into the genome, e.g. horizontally transferred elements, will also be less similar to the other windows in its genome. As a threshold, we select those windows/genes having an R2 value two standard deviations from the genome average R2 value as exhibiting unusual compositional properties. Of the 18 genomes examined here, no windows/genes had an R2 value greater than or equal to two standard deviations greater than the genome average. Given our interest in regions acquired from foreign sources, putative virulence factors and genes under strong selection (despite genome compositional proclivities), genes such as rRNA were not classified as RUCPs. We refer to the regions exhibiting unusual compositional properties from the window-by-window analysis as wRUCPs and from the gene-by-gene analysis gRUCPs.
The source code, including fixed and variable window functionality implemented in C++, is freely available upon request.
Visualizing similarity and dissimilarity between genomes
To visualize the similarity, we plot the matrix of correlation coefficients (r), C(i,j), between the distributions of k-mers, where i is a window in the first genome and j is a window in the second genome. (Note, visualization is performed using r values not R 2 values in an effort to expand the spectrum of values observed). The vertical and horizontal coordinates in an S-plot represent the location of windows i and j, respectively. Different correlation coefficients are represented on the plots by different colors. An application to generate S-Plots using the C# language for Windows was created and is freely available at http://www.bioinfo.uh.edu/splot. For further details, the reader is referred to  and the online documentation accompanying the application. The gene-by-gene functionality is not included within the visualization tool.
Comparative analysis of RUCPs
wRUCPs and gRUCPs were firstly compared to the other members of the Neisseria spp. as well as all publicly available genomes. Similarities between Neisseria spp. were conducted by examining correlations between each individual RUCP and all windows in the other Neisseria sequences. Sequences were either aligned using BioEdit (http://www.mbio.ncsu.edu/bioedit/bioedit.html) or BLASTed. BLAST-based analysis was performed as follows. For each RUCP, be it a window or an individual gene, the sequence was parsed from the complete genome. Using the BLAST web interface, each sequence was compared to all sequences within the nucleotide collection nr/nt database using BLASTn with a word size of 7. In addition, the search was conducted with the exclusion of the taxonomical group for Neisseria (taxid: 482) and an e-value threshold of 0.5. Upon inspection of the results for each sequence, only those hits meeting the aforementioned threshold and resulting in a query coverage greater than 50% were considered homologous. Genes which uniquely appeared in a single Neisseria strain were also compared to all publicly available genomes in the nucleotide collection nr/nt database. The same threshold, e-value and exclusion as our previous BLAST analysis were applied again here. BLASTx searches were also conducted to examine the similarity of gRUCPs conserved amongst all pathogenic Neisseria spp., amongst all N. meningitidis spp., and amongst all N. gonorrhoeae spp. with other Neisseria sequences which were not included in this analysis as well as other bacterial genomes. A word size of 2 was used for more sensitive searches. All e-values less than 1.0 were considered putative hits.
The authors would like to thank Audrey Hart-van Tassell, Petri Urvil, Meizhuo Zhang, Sergei Chumakov for their contributions in the initial efforts in this project. MS was partially supported through Loyola University Chicago’s Biology Summer Research Fellowship.
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