Contrasted evolutionary histories of two Toll-like receptors (Tlr4 and Tlr7) in wild rodents (MURINAE)

Table 1 Estimates of sequence diversity and average codon-based evolutionary divergence over all sequence pairs for the exon 3 and particular domains of Tlr 4 and Tlr 7 genes

*Tlr* domains	n	L	π±S.E.	hN	hA	S	*Eta*	*dN±*S.E.	dS±S.E.	*dN/* dS
*Tlr* 4
Exon 3	96	2247	0.049±0.003	122	90	545	625	0.038±0.003	0.102 ±0.008	0. 481
ECD	96	1647	0.053±0.003	112	83	441	504	0.045±0.004	0.098 ±0.009	0. 597
LBR	96	666	0.072±0.006	67	50	203	242	0.070±0.008	0.108±0.015	0. 787
TIR	96	435	0.031±0.002	54	11	68	79	0.004±0.002	0.143 ±0.024	0. 067
*Tlr* 7
Exon 3	96	3147	0.034±0.003	79	49	466	518	0.021±0.002	0.088 ±0.007	0. 398
ECD	96	2547	0.037±0.003	75	48	407	455	0.025±0.002	0.089±0.007	0. 468
LBR	96	311	0.035±0.003	19	8	37	38	0.018±0.006	0.107±0.024	0. 196
TIR	96	420	0.026±0.003	26	6	43	47	0.007±0.003	0.105±0.021	0. 070

Note. - ECD extracellular domain, LBR - ligand biding region, TIR Toll/interleukin-1 receptor domain, n the number of sequenced individuals, L length of analysed sequences in base pairs, π average number of nucleotide differences per site between two sequences, S. E. Standard error, hN number of nucleotide alleles, hA number of amino acid variants, S number of polymorphic sites, Eta total number of mutations, dS number of synonymous substitutions per synonymous site (estimated by MEGA), dN number of non-synonymous substitutions per non-synonymous site (estimated by MEGA). Analyses were conducted using the Nei-Gojobori model; S.E. of dN and dS - were obtained by a bootstrap procedure (1000 replicates); dN/dS were computed by SLAC (Datamonkey).

ISSN: 2730-7182