Figure 6

Exons encoding the coiled-coiled regions of Liliopsida myosin-11E1 and myosin-11E2. A) The Liliopsida subtype 11E1 and 11E2 myosins contain clusters of exons with identical lengths, reading frame, splice sites, and sequence similarity similar to mutually exclusive spliced exons. However, cDNA clones are available spanning the first few exons of the clusters indicating that all exons of the cluster are most probably constitutively spliced. Here, the gene structures of Oryza sativa japonica group (Os_a) myosin-11E1 and myosin-11E2 are shown as examples. The clusters of similar exons span exons 25–36 of myosin-11E1 and exons 25–40 of myosin-11E2. Exons are denoted as dark-grey and introns as light-grey bars. The similar exons of the clusters are coloured in green. Sequence similarity of the corresponding exons is shown in the multiple sequence alignments below the respective gene structures. B) The phylogenetic tree of exons 25–36 of the 11E1 variant and exons 25–40 of the 11E2 variant shows that the exons of both variants correspond to each other with the exception of exons 26, 30, 31 and 35 of variant 11E2. Phylogenetic trees were calculated with RAxML (shown), MrBayes and ClustalW, and bootstrap values (in percentages) and posterior probabilities for the nodes are given.