Figure 3

Positive selection within Pgi and Gapdh . Distribution of positively selected sites across three Lanceocercata stick insect genes: phosphoglucose isomerase 1 (Pgi1), phosphoglucose isomerase 2 (Pgi2) and glyceraldehyde 3-phosphate dehydrogenase (Gapdh); Probability of each codon position being under positive selection (ω > 1) using Bayes Empirical Bayes analysis under the M8 site-specific model in PAML. The Pgi1 analysis consists of 225 codons from 28 species, Pgi2 484 codons from 20 species and Gapdh 279 codons from 29 species. Codon position based on full-length alignment of both Pgi genes from Micrarchus nov. sp. 2. Amino acid sites identified in previous studies as either under positive selection, associated with fitness differences or segregating between species for PGI are shown. The secondary structure of each protein in relation to codon position is shown.