Table 3 Information for the 18 Y-SNP markers and primer sequences
Marker | Amplicon size (bp) | PCR primer sequnces (5’→3’) | Test method | |
|---|---|---|---|---|
Forward | Reverse | |||
C/M216 | 109bp | TCACTTTTATATCCTCAACCA | AATCTGAATTCTGACACTGC | Sequence |
C3/M217 | 101bp | ACTTGTGAAGGAGAATGAAAA | GCATTTGATAAAGCTGCTGTG | Sequence |
C3c/M48 | 140bp | AAACAATATGTATGCTAATTTTGCT | TCAATGTAAATGTTAGTATAAGGATG | Sequence |
C3d/M407 | 114bp | TCTTACTGAAAGTTGGGGAC | GATAATCGCTTGTCTCTTGG | Sequence |
C3e/P53.1 | 123bp/121bp | AAACCCTGGAGAGGACCAACGA | ACACTATGAACCAATCCCACCC | Sequence |
F/M89 | 125bp | CCACAGAAGGATGCTGCTCA | CACACTTGGGTCCAGGATCAC | Sequence |
K/M9 | 128bp | GGACCCTGAAATACAGAAC | AAGCGCTACCTTACTTACAT | Sequence |
P/M45 | 129bp | GGGTGTGGACTTTACGAAC | AAATCCTACTATCTCCTGGC | Sequence |
NO /M214 | 119bp | MACTGGAAAGAAAAAGAATGCTG | ATGGAAATGCCACTTCACTC | Sequence |
N/M231 | 113bp | CCTGGAAAATGTGGGCTC | TTCTTTGACGATCTTTCCCC | Sequence |
N1a/M128 | 123bp/121bp | ATCTACCTCTTTCAAACTGT | GAACTGCCTCTTATAAAATCAT | Sequence |
N1b/P43 | 108bp | ACGGAGTCTCGCTCTGTCG | GCTACTTGGGAGGCTGAGG | Sequence |
N1c/Tat | 115bp | GAGAAGGTGCCGTAAAAGTG | GGACTCTGAGTGTAGACTTGTG | Sequence |
O/M175 | 110bp/105bp | TCAACTCAACTCCAGTGATTTA | TAATGATACCTTTTTTTCTACTGA | PAGE |
O3/M122 | 120bp | CAGATACTGTGACTTTGAG | GAAATGAATAAATCAAGGT | Sequence |
O3a/M324 | 120bp | GGAACATAGCAAGACCCAAAAT | CAAATTGATTTCCAGGGATACAT | Sequence |
O3a3/P201 | 114BP | FTAAGCAATGAAGGTAGAAGG | ATTTAGAATAATATTTACTGAGCA | Sequence |
O3a3c/M117 | 117bp/113bp | AAAATAACTCACCAAAGGAAT | AGATGATAGAAAAACATAATA | PAGE |