Figure 6

Chronogram of Heterandria formosa and related poeciliid species diversification, and gene tree results. A) Chronogram resulting from Bayesian relaxed-clock coalescent-dating analysis in BEAST based on mitochondrial cytb and RPS7 variation. Tip labels are sequence codes including population, site number, and specimen code for each individual sequenced (details in Figure 1 and Table 1). Node bars (dark blue) are 95% highest posterior densities for node ages. The analysis included three lognormally modeled fossil/biogeographic calibration points (red triangles enclose bounds of constraints). Mean node ages of interest are discussed in detail in the text. Nodal support values are of the form: Bayesian posterior probabilities (PP; ≥95)/maximum-likelihood bootstrap proportions (BP; ≥50). B) Comparison between the 'best’ gene tree of H. formosa cytb haplotypes and the 'Minimize Deep Coalescences’ species tree (at right) inferred from the haplotype tree using Maddison and Knowles’ [76] method, with BP ≥ 50 by each node.