Figure 4

Phylogenetic tree showing the relationship between hydrophobins from a representative set of ascomycetes. The tree was inferred using the Neighbor-Joining method. The optimal tree with the sum of branch length = 23.48723281 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown above the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the JTT matrix-based method and are in the units of the number of amino acid substitutions per site. The analysis involved 59 amino acid sequences. All ambiguous positions were removed for each sequence pair. There were a total of 136 positions in the final dataset. Three clades Q (purple), R (blue) and S (green) are represented in the tree. Clades R and S = monophyletic groups. Clade Q = class I hydrophobins from ascomycetes with mixed lifestyles, pathogens, saprotrophs and mycoparasites. Clades R = class II hydrophobins from Trichoderma species. Clade S = class II hydrophobins from Aspergillus species except one sequence from the rice blast fungus, M. grisea. Fungal species are indicated with the following abbreviations. |Thite2|, Thielavia terrestris; |Acral2|, Acremonium alcalophilum, |Aspca3|, Aspergillus carbonarius, |Aspcl1|, Aspergillus clavatus; |Aspnid1|, Aspergillus nidulans; |Dotse1|, Dothistroma septosporum, |Lepmu1|, Leptosphaeria maculans, |Triat2| Trichoderma atroviride, |TriviGv|, Trichiderma virens, |Altbr1|, Alternaria brassicicola, |Triha1|, Trichoderma harzianum, |Pench1|, Penicillium chrysogenum, |Neudi1|, Neurospora discreta, |Fusgr1|, Fusarium graminearum, |Fusox1|, Fusarium oxysporum, |Maggr1|, Magnaporthe grisea, |Neucr1|, Neurospora crassa, |Neute_mat_a1|, Neurospora tetrasperma, |Verda1|, Verticillium dahliae.