Figure 4

Functional assessment of positively selected sites from Myotis lucifugus by comparison to human orthologs. In both (A) and (B) the amino acid position of the human gene is given on the x-axis and the PP of a given site being under positive selection is given on the y-axis. (A) are the results for DKC1 and (B) are the results for TERT. Positively selected sites are shown as red dots throughout and different domains are denoted with roman numerals and are highlighted by blue semi-transparent blocks. For (A) blue shaded regions are: (i) nucleolar localization, (ii) PUA domain, and (iii), nuclear and nucleolar localization respectively. In (B) mutagenesis sites which result in reduced telomerase activity are represented by vertical green lines, and blue shaded regions are: (i) RNA-interacting domain 1, (ii) Required for regulating specificity for telomeric DNA, (iii) Required for oligomerization, (iv) RNA-interacting domain 2, (v) Reverse transcriptase, and (vi), Required for oligomerization. The purple vertical lines in (A) represent the natural variants resulting in XDKC, and the vertical green lines in (B) represent the position of the natural variants resulting in HH0053.