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Table 4 Polymerase mistake versus heteroplasmy test

From: Dugesia sicula (Platyhelminthes, Tricladida): the colonizing success of an asexual Planarian

Specimen

Direct sequence (heteroplasmic positions)

N cloned sequences

N bp

Expected point mutations

Observed point mutations

Poisson P[X < obs]

s23.02

Haplotype A

8

4296

2.7

1

0.07

s23.09

Haplotype A

7

3759

2.3

1

0.10

s27.14

Haplotype B

11

5907

3.7

14

1.00*

s27.19

Haplotype B

11

5907

3.7

11

1.00*

s36.12

Haplotype B

10

5370

3.4

7

0.95

s36.03 1

Haplotype A

10

5188

3.0

4

0.64

s39.08

Haplotype A

7

3759

2.3

6

0.97

s39.11

Haplotype A

10

5370

3.4

7

0.95

s39.14

Haplotype B

10

5370

3.4

4

0.57

s42.10

Haplotype B

9

4833

3.0

34

1.00*

s06.07

Heteroplasmic (2)

8

4296

2.7

33

1.00*

s26.12

Heteroplasmic (1)

10

5370

3.4

31

1.00*

s31.18

Heteroplasmic (10)

29

15530

9.7

219

1.00*

s31.20

Heteroplasmic (6)

9

4833

3.0

63

1.00*

s33.10

Heteroplasmic (6)

4

2148

1.3

5

0.99*

s47.03 1

Heteroplasmic (3)

9

4692

2.7

71

1.00*

s47.11 1

Heteroplasmic (5)

10

5088

2.7

32

1.00*

s48.04

Heteroplasmic (4)

12

6444

4.0

95

1.00*

s48.10

Heteroplasmic (2)

10

5370

3.4

4

0.57

s55.10

Heteroplasmic (4)

3

1611

1.0

8

1.00*

s56.15

Heteroplasmic (7)

7

3759

2.3

26

1.00*

  1. Expected number of point mutations for each individual based on a polymerase error rate of 6.3 × 10-4[28], point mutations found in cloned sequences, and probability that observed point mutations exceed the expected based on a Poisson distribution.
  2. Sequence length was 537 bp, except in some cases marked with 1; N bp, total length amplified (length amplified x number of clones); *Significant differences based on 99% criterion.